中文摘要：

[目的]建立同时测定西瓜和土壤中戊唑醇、肟菌酯及代谢物肟菌酸残留量的超高效液相色谱串联质谱法（UPLC-MS／MS）。[方法]样品中的待测农药组分采用乙腈-水（体积比4：1）提取，硅藻土液液萃取小柱净化，采用UPLC-MS／MS法，ESI（＋）电离方式，多反应监测（MRM）定量测定。[结果]仪器最低检测量为戊唑醇5．0×10^-13g，肟菌酯4．0×10^-13g，肟菌酸2．5×10^13g；3种农药的最低检出质量分数均为0．02mg／kg，在西瓜和土壤中的添加回收率为81．8％-94．2％，变异系数为3．67％-7．04％。[结论]该方法快速、准确、灵敏度高，适合西瓜与土壤样品中戊唑醇、肟菌酯及肟菌酸的残留量测定。

英文摘要：

[Aims] A method was developed for the simultaneous determination of tebuconazole, trifloxystrobin and its metabolite trifloxystrobin acid in watermelon and soil by ultra performance liquid chromatography tandem mass spectrometry （UPLC-MS/MS） detection. [Methods] The pesticides were extracted using mixed solvent of acetonitrile and water （4 ： 1, by vol）, and were separated and purified with column of diatomite liquid liquid phase extraction. The pesticides were finally detected by UPLC-MS/MS-ESI（＋）, quantified with multiple reaction monitoring（MRM） mode. [Results] The limit of detection （LOD） was 5.0×10^-13g for tebuconazole, 4.0×10^-13g for trifloxystrobin, 2.5×10^-13 g for trifloxystrobin acid, while the quantification limit （LOQ） was 0.02 mg/kg for three compounds. The average recoveries of tebuconazole, trifloxystrobin and trifloxystrobin acid were 81.8-94.2%, with relative standard deviation （RSD） of 3.67-7.04% in watermelon and soil. [Conclusions] It is an effective method which attained the pesticide residue analysis demands in sensitivity, accuracy and precision for detecting residues of tebuconazole, trifloxystrobin and trifloxystrobin acid in watermelon and soil.