中文摘要：

以番茄为材料,采用RT-PCR技术从番茄果实中克隆了其体内维生素E合成相关基因—生育酚环化酶（Tocopherol cyclase VTE1）的部分序列,片段长420bp,序列分析表明,该基因片段与番茄中生育酚环化酶cDNA序列同源性为95%,然后通过XhoI、KpnI 2个酶切位点连接VTE1片段和含PDS的pTRV2质粒,获得了重组载体pTRV2-PDS-VTE1,将该重组载体转化农杆菌GV3101,并侵染番茄。该研究为下一步分析基因沉默后的番茄中维生素E含量的变化提供试验材料;为今后通过转基因技术调节番茄果实的维生素E的合成奠定了基础。

英文摘要：

Tomato was used as test material,using RT-PCR to clone part sequence of vitamin E biosynthesis gene＇s（tocopherol cyclase VTE1） from the tomato fruit.Fragment length was 420 bp.Sequence analysis showed that gene homology of this fragment and VTE1 was 95%.Then XhoI,KpnI restriction sites connected VTE1 with pTRV2-PDS plasmid.The recombinant vector pTRV2-PDS-VTE1 was obtained.And the recombinant vector was transformed into Agrobacterium.The study provided the experimental material for using VIGS technology to further analysis VTE1＇s role in vitamin E biosynthesis in tomato.On this basis,laid the foundation for using transgenic technology to control vitamin E biosynthesis in tomato fruit in the future.