欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
建立反相离子对-超高效液相色谱（RPIP-UPLC）和电喷雾离子源-四极杆-飞行时间质谱（ESI-Q-TOF-MS）联用技术快速分离鉴定硫酸寡糖的方法。以20mmol/L庚胺（pH4）为离子对试剂,25%庚胺甲酸盐纯水溶液（A）和25%庚胺甲酸盐甲醇溶液（B）为梯度洗脱溶剂,κ-卡拉胶寡糖通过BEHC18反相柱分离后,分别在正、负离子模式下进行四极杆-飞行时间质谱分析。结果表明,聚合度为3～45的κ-卡拉胶寡糖在BEH C18柱上得到很好的分离,从每一个色谱峰对应的质谱图中可以准确获得直至27糖的各寡糖结构信息,均为奇数糖,与聚丙烯凝胶电泳结果吻合。所得的寡糖断裂规律对卡拉胶寡糖的快速鉴定和结构解析具有重要意义。
英文摘要：
A method was developed to elucidate the structures of sulfated oligosaccharides through establishment of an effective reversed phase ion pair ultra-performance liquid chromatography coupled with electrospray ionization time of flight mass spectrometry（RPIP-UPLC-ESI-TOF-MS）. Heptylamine （20 mmol/L, pH 4） has been selected as the ion-pairing agent. κ-Carrageenan oligosaccharides have been separated on BEH Cls column using MeOH/H20 with 25% heptylammonium formate as eluent in linear gradient mode. Mass spectra were obtained by ESI-Q-TOF-MS in both positive and negative modes, κ-Carrageenan oligosaccharides were well separated up to pentatetrasaccharide, and ESIMS analysis for κ-carrageenan oligosaccharides up to heptacosasccharide. The results showed that all acid hydrolyzed κ-carrageenan oligosaccharides were odd sugars, which was further confirmed by polyacrylamide gel electrophoresis （PAGE）. The characteristic fragmentation pattem of ion-pair oligosaccharides in mass spectra can be applied for rapid structure identification.
同期刊论文项目
同项目期刊论文
lambda-Carrageenan oligosaccharides elicit reactive oxygen species production resulting in mitochond
Analysis of Oligo-kappa-carrageenan by Reversed Phase Ion-pair Ultra-Performance Liquid Chromatograp
期刊信息
位置：