中文摘要：

目的 采用高通量蛋白质组学筛选鉴定小鼠心肌原代细胞中可能与心肌肌钙蛋白I（c Tn I）存在相互作用的蛋白质并通过生物信息学软件预测其可能参与的生物过程及信号通路。方法 取C57新生小鼠的心脏制备心肌原代细胞,提取细胞全蛋白行免疫共沉淀实验筛选出c Tn I的相互作用蛋白,通过质谱分析技术对其进行鉴定;将鉴定所得蛋白质信息输入生物信息学软件分析其理化性质,预测其可能参与的生物过程及信号通路。结果 共鉴定出262种可能与c Tn I存在相互作用的蛋白质,预测其可能参与的生物进程14条,KEGG生物学通路33条（P〈0.05）。结论 在小鼠心肌细胞中,有262种蛋白可能与c Tn I存在相互作用,其中部分蛋白可能参与心肌细胞钙离子内流及PI3K-Akt信号通路的调节。

英文摘要：

Objective To isolate and identify the proteins that interact with cardiac troponin I （cTnI） in primary mouse cardiomyocytes, and to analyze their biological processes and signaling pathways by use of High throughput proteomics and bioinformatics techniques. Methods The hearts of C57 neonatal mice were used to prepare the primary cardiomyocytes. The cTnI binding proteins were extracted from the whole cell protein by co-immunoprecipitation, and the proteins were analyzed and identified by mass spectrometry. Furthermore, the physicochemical properties, biological processes and signaling pathways of cTnI interacting proteins were predicted by bioinformatics tools. Results A total of 262 proteins were identified by mass spectrometry, cTnI binding proteins were involved in 14 biological processes and 33 KEGG biological pathways （P〈0.05）. Conclusion In mouse cardiomyocytes, 262 kinds of proteins may interact with cTnI, and participate in regulation of calcium ion influx and PI3K-Akt signaling pathways.