中文摘要：

基于河南省动物免疫学重点实验室制备并保存的莱克多巴胺单克隆抗体（RAC McAb）,采用异硫氰酸荧光素（FITC）作为标记材料对之进行荧光标记,并对荧光探针FITC-RAC McAb进行纯化和荧光光谱鉴定;应用免疫层析技术研制RAC残留荧光检测试纸,并对其敏感性、特异性、重复性进行初步测定。结果表明：荧光探针标记成功,并应用于免疫层析试纸,检测试纸的最低检测限为1μg/L,较同抗体的胶体金试纸的敏感性提高10倍;与其他激动剂类药物无交叉反应,具有良好的特异性;以不同批次的检测试纸对猪尿样和牛奶样进行检测,结果一致,具有良好的重复性;检测试纸在10min内可显示结果。

英文摘要：

Monoclonal antibody against ractopamine,which was prepared and stored by Henan Provincial Key Laboratory of Animal Immunology,had been labeled with fluorescein isothiocyanate（FITC）.The fluorescence probe（FITC-RAC McAb） was purified and characterized by fluorescence spectrophotometer.Based on the immunochromatographic technology,the fluorescence test strip of RAC was developed and its sensitivity,specificity and repeatability were tested respectively.The result showed that the fluorescence probe had been successfully prepared,the eyeballing detection limit of the test strip was 1 μg/L,which was 10 times higher than that of colloidal gold test strip with the same McAb.It generally had no cross reactivity with other agonist.Swine urine samples and milk samples was detected with the test strip of different batches,the results showed that the test strip had good reproducibility and could give the test results in less than 10 min.