中文摘要：

靶向基因-病毒治疗方法是近年来产生的一种较为有效的癌症生物治疗方法。但其癌症治疗效果仍需进一步提高。在该研究工作中，通过联合使用临床神经治疗药物硫利达嗪（thior_idazine）与溶瘤腺病毒ZD55-TRAIL来增强对HeLa细胞的杀伤作用。通过MTT实验、倒置显微镜观察、结晶紫染色实验观察了联合使用thioridazine和ZD55-TRAIL对子宫颈癌细胞株HeLa细胞的毒性作用；使用Hoechst33342染色、流式细胞实验和Western blot实验检测了联合使用thioridazine和ZD55-TRAIL在引起HeLa细胞发生凋亡上的作用。结果表明，小分子药物thioridazine与病毒ZD55-TRAIL联合使用可以增强对HeLa细胞的杀伤作用，通过下调抗凋亡蛋白XIAP的水平，更显著地促进HeLa细胞发生凋亡。该研究首次报道了联合使用精神疾病药物thioridazine和ZD55-TRAIL对子宫颈癌细胞HeLa的抑制作用，可能是子宫颈癌治疗的一种有效方法。

英文摘要：

Targeting Cancer Gene-Virotherapy is a newly developed method for cancer therapy. However, its efficiency still needs to be improved. In this study, we enhanced the cytotoxicity on HeLa cells with the combined use of clinical antipsychotic drug thioridazine and ZD55-TRAIL. Cytotoxicity of the combination of thioridazine and ZD55-TRAIL on HeLa cells was examined by MTT assay, direct observation and crystal violet staining. Hoechst33342 staining assay, flow cytometry experiments and Western blot assay were used to detect the occurrence of apoptosis in HeLa cells with the treatment of thioridazine and ZD55-TRAIL. We found that small molecule drug thioridazine combing with the virus ZD55-TRAIL could enhance the killing effect on HeLa cells. In addition, the results showed that the combined treatment led to lower level of anti-apoptotic XIAP protein and apoptosis was more obvious. This work firstly reported the inhibition effect of the combination of the antipsychotic drug thioridazine and ZD55-TRAIL on HeLa cells, and it could be a potential therapeutic approach for cervical cancer therapy in the future.