中文摘要：

目的 观察白细胞介素（IL）-17、白细胞介素（IL）-4及γ干扰素（IFN-γ）在实验性自身免疫性葡萄膜炎（EAU）中的表达.方法 采用健康雌性C57BL/6小鼠建立EAU模型.建模前及建模后7、14、21、28 d,取小鼠眼球作石蜡病理切片,光学显微镜观察小鼠视网膜结构变化;采用酶联免疫吸附试验检测小鼠血清中IL-17、IL-4、IFN-γ含量;采用实时荧光定量聚合酶链反应检测小鼠视网膜组织中IL-17、IL-4、IFN-γ的mRNA表达;采用蛋白质免疫印迹法检测小鼠视网膜组织中IL-17、IL-4、IFN-γ的蛋白表达.结果 建模后14d,光学显微镜观察发现,葡萄膜组织结构破坏,大量炎症细胞浸润.小鼠血清中IFN-γ含量,脾及视网膜组织中IFN-γ mRNA和蛋白表达均于建模后7d达到高峰;小鼠血清中IL-4、IL-17含量,脾及视网膜组织中IL-4、IL-17 mRNA和蛋白表达均于建模后14 d达到高峰.小鼠血清中IL-17含量,脾及视网膜组织中IL-17 mRNA和蛋白表达升高程度均较IFN-γ、IL-4更为显著.建模前与建模后7、14、21 d小鼠血清中IL-17、IL-4、IFN-γ含量比较,差异均有统计学意义（F=1 817.346、268.600、164.621,P＜0.05）;建模前与建模后28d小鼠血清中IL-17、IL-4、IFN-γ含量比较,差异无统计学意义（P＞0.05）.建模前与建模后7、14、21d小鼠脾（F=312.67、114.250、216.220）及视网膜（F=271.504、85.370、80.722）组织中IL-17、IL-4、IFN-γ蛋白表达比较,差异有统计学意义（P＜0.05）;建模前与建模后28 d小鼠脾及视网膜组织中IL-17、IL-4、IFN-γ蛋白表达比较,差异无统计学意义（P＞0.05）.结论 IL-17、IL-4、IFN-γ在EAU中均明显表达,共同参与了EAU疾病的过程.

英文摘要：

Objective To observe the expression of interleukin （IL）-t7,IL-4 and interferon γ （IFN-γ） in experimental autoimmune uveoretinitis （EAU）.Methods C57BL/6 mice were immunized with interphotoreceptor retinoid-binding protein 1-20 to induce EAU.The inflammatory reaction before and on 7,14,21,28 days after immunization were observed.The level of IL-17,IL-4 and IFN-γ in the serum were measured by enzyme-linked immune sorbent assay （ELISA）.mRNA and protein expression of spleen and retina were analysed using quantitative reverse transcription-polymerase chain reaction （RT-PCR） and Western blot at the same time,respectively.Results The most serious inflammatory reaction occurred at the 14th day after immunization.The highest level of IFN-γ in serum,highest mRNA and protein expression of IFN-γ in spleen and retina of mice occurred at day 7 after being immunized.The highest level of IL-17,IL-4 in serum,highest mRNA and protein expression of IL-17,IL-4 in spleen and retina of mice occurred at day 14 after being immunized.The increase degree of IL-17 was more than IFN-γ and IL-4.At 7,14 and 21 days after immunization,compared with the pre-immunization,the level of IL-17,IL-4,IFN-γ in serum of mice were significantly increased （F=1 817.346,268.600,164.621; P＜0.05）.There was no difference in the levels of IL-17,IL-4,IFN-γin serum of mice between pre-and 28 days after immunization （P＞0.05）.At 7,14 and 21 days after immunization,compared with the pre-immunization,the protein expression of IL-17,IL-4,IFN-γ in spleen （F=312.67,114.250,216.220） and retina （F=271.504,85.370,80.722） of mice were significantly increased （P＜0.05）.There was no difference in protein expression of IL-17,IL-4,IFN-γ in spleen and retina of mice between pre-and 28 days after immunization （P＞0.05）.Conclusions There were IL-17,IL-4 and IFN-γ expression in EAU.IL-17,IL-4 and IFN-γplay a key role in the occurrence and development of the EAU.