中文摘要：

为了准确测定家兔血液中的N-亚硝基降烟碱（NNN）及其代谢物,通过对色谱、质谱条件的优化和选择,建立了以NNN-d4为内标,通过沉淀蛋白的前处理操作以及Waters亲水相互作用色谱（HILIC）柱分离和多离子反应监测模式（MRM）测定大耳朵白兔血浆中NNN及其代谢物的HPLC-MS/MS方法。结果表明,NNN及其代谢物的相对标准偏差（RSD）、回收率和检测限分别为0.50%~8.62%,80%~111%和0.039~0.217 ng/mL。该方法简单、快速灵敏,适合于血液中NNN及其代谢物的检测。

英文摘要：

In order to accurately determine N-nitroso nornicotine（NNN） and its metabolites in rabbit blood,a high-performance liquid chromatography/tandem mass spectrometry method was established by optimizing chromatographic conditions and mass spectrometric conditions and using NNN-d4 as an internal standard.The samples were successively pretreated by protein precipitation,separated by a Waters-HILIC column,and analyzed by tandem mass detector under multi-reaction monitoring（MRM） mode.The results showed that the recoveries of NNN and its metabolites ranged from 80% to 111% with the relative standard deviations（RSDs） of 0.5% to 8.62% and the limits of detection of 0.039 to 0.217 ng/mL.This method is simple,sensitive,rapid,and suitable for the analysis of NNN and its metabolites in blood.