中文摘要：

本文以上转换发光材料（UCPs）为供体,聚间苯二胺（PMPD）为受体,构建了基于荧光共振能量转移（FRET）的传感平台,并将其用于凝血酶的检测。一定浓度的PMPD加入到标记单链DNA的UCPs体系中,对上转换发光的猝灭效率可以达到70%。当加入浓度在0.2~5.0nmol/L范围内的目标物凝血酶时,其荧光恢复程度与浓度呈线性关系,检出限为0.18nmol/L。PMPD因其良好的水溶性、荧光猝灭能力及免标记的特点为基于FRET技术的生物样品检测提供了新的平台。

英文摘要：

In this study,a new biosensor for the detection of thrombin based on upconversion fluorescence resonance energy transfer （UC-FRET） from upconversion phosphors （UCPs） to poly-m-phenylenediamine （PMPD） was contructed. With the rr- rr stacking between PMPD and thrombin aptamer which was covalently coupled with polyacrytic acid modified UPSs, the fluorescence of UCPs was quenched with a maximum quenching degree of 70%. When thrombin was introduced, the aptamer was preferentially combined with thrombin, which blocked the FRET between UCPs and PMPD. The fluorescence of UCPs was accordingly recovered. And the fluorescence restoration of UCPs obtained was linearly related to thrombin concentration ranging from 0.2 to 5.0 nmol/L. A detection limit of 0. 18 nmol/L in aqueous solution was acquired. Good quenching ability, water solubility and label-free property ensure PMPD as a potential and perfect acceptor. The UC-FRET biosensor afforded a new favorable method for the detection of thrombin.