中文摘要：

为调控生物活性Ti金属材料的生物活性,使用灭活的细菌生物膜对其进行杂化。金黄色葡萄球菌（S.aureus）在纯Ti（P-Ti）和酸碱处理Ti（AA-Ti）表面培养5 d后在121℃、0.13 MPa条件下灭活30 min,获得细菌生物膜杂化的纯Ti（BP-Ti）和酸碱处理Ti（BAA-Ti）。BCA蛋白分析和苯酚-硫酸分析表明,在BAA-Ti表面的细菌生物膜的细胞外基质较BP-Ti表面细菌生物膜的细胞外基质含较多蛋白,而后者含有较多的多糖。酶免疫分析表明,在BP-Ti和BAA-Ti表面的肠毒素都低于阈值。模拟体液（SBF）浸泡实验表明,BAA-Ti和BP-Ti在5 d内均可诱导磷灰石形成,但BAA-Ti诱导磷灰石能力低于BP-Ti。AA-Ti在1 d内可诱导磷灰石生成,但在5 d内P-Ti表面无磷灰石生成。表明生物膜提高了BP-Ti的生物活性,但降低了BAA-Ti的生物活性。这可能源于生物膜中多糖有利于促进磷灰石的形成,而其中的蛋白抑制磷灰石的形成。MG-63细胞培养实验表明,

英文摘要：

Bacterial biofilm contained with proteins and polysaccharides, which made it have the potential to mimic extracellular matrix for tissue repair. It also has the properties to attach the substrate firmly.In order to regulate the bioactivity of titanium metal, inactivated bacterial biofilm was hybridized on the metal surfaces. Staphylococcus aureus（S.aureus） was cultured for 5 d on pure titanium metal（P-Ti） and thatsubjected to acid-alkali treatment（AA-Ti）, and then the bacteria was inactivated at 121 ℃ and 0.13 MPa for 30 min to get hybridized titanium metals BP-Ti and BAA-Ti respectively. BCA（bicinchoninic acid） assay and phenol-sulfuric acid analysis showed the extracellular matrix（ECM） in the biofilm on BAA-Ti contented less polysaccharide and more protein than that on BP-Ti. Enzyme immunoassay showed the staphylococcal enterotoxins in both biofilms were lower than the threshold value. SBF（simulated body fluid） soaking experiments showed both BAA-Ti and BP-Ti could induce apatite formation after 5 d, and BAA-Ti induced less apatite than the BP-Ti did. The AA-Ti could induce apatite formation at 1 d, but there is no apatite formation on P-Ti even after 5 d. It indicated that the biofilm decreased the bioactivity of BAA-Ti, but increased the bioactivity of BP-Ti. This effect might depend on the roles of proteins and polysaccharide in the biofilms which could restrain the apatite formation for the former, and accelerate the apatite formation for the later. When osteosarcoma cell MG-63 was cultured on the metals for 3 d, the cell proliferation ability on the metals followed by the order of BAA-TiBP-TiAA-TiP-Ti, which indicated that the proteins might inhibit the cell proliferation. The biofilms hybridized on titanium metal could influence the biological response of titanium metal. It might be possible to regulate the bioactivity of titanium by biofilm hybridization.