中文摘要：

目的：应用现代分析检测技术,定量分析附子与半夏、瓜蒌、贝母、白蔹、白及配伍前后水煎液中3种双酯型生物碱（乌头碱、中乌头碱、次乌头碱）,考察其含量变化的影响因素。方法：采用高效液相色谱法,Agilent Ex-tend C18柱,乙腈-甲醇（V∶V=1∶1）-35 mmol/L乙酸铵梯度洗脱,流速0.6 mL/min,检测波长235 nm。结果：除川贝母、白蔹、法半夏外,其他各药与附子配伍后煎煮液中3种双酯型生物碱均高于附子单煎液,而其变化趋势与煎煮液的pH值有联系。结论：共煎液的pH值是影响药液中3种双酯型生物碱含量的直接因素。

英文摘要：

Objective： To analyze the three diester-alkaloids content in the decoctions before and after Radix aconiti lateralis preparata combined with Rhizoma pinelliae,Fructus trichosanthis,Bulbus fritillariae,Radix ampelopsis,Rhizoma bletillae,respectively.Methods： HPLC analysis was performed on a Agilent Extend C18 column,eluted with a mobile phase consisted of acetonitrile/ methanol-35 mmol/L ammonium acetate and gradient elution,with a flow rate of 0.6 mL/min and the detection wavelength of 235 nm.Results： The contents of three diester-alkaloids in the co-decoctions of Radix aconiti laterlis preparata combined with Rhizoma pinelliae,Fructus trichosanthis,Bulbus fritillariae,Rhizoma bletillae were more than that of single Radix aconiti laterlis preparata decoction,expect the Bulbus fritillariae cirrhosae,Radix ampelopsis and Rhizoma pinelliae praeparatum.From the result,we can found that the content of three diester-alkaloids in decoctions was correlated with the decoction pH.Conclusion： The pH of decoction is one of the most important factors to relate the three diester-alkaloids content in the decoctions.