中文摘要：

以烟草悬浮细胞BY-2为材料,探讨了在Al Cl3胁迫下交替氧化酶对烟草悬浮细胞死亡发生的影响.结果表明,随着Al Cl3的增加（30、50、80、100μmol/L）,烟草悬浮细胞的细胞死亡水平逐渐上升,交替呼吸途径的容量水平随之增大.在Al Cl3胁迫下细胞中H2O2的浓度也有所增加.较之在Al Cl3处理下的细胞,将交替氧化酶抑制剂水杨基氧肟酸预处理后的细胞再置于Al Cl3的胁迫下,则导致了细胞死亡水平和H2O2含量的进一步上升;H2O2可以以剂量依赖的方式导致细胞死亡水平的上升,H2O2的清除剂可以降低Al Cl3胁迫及H2O2处理下细胞死亡的水平.在Al Cl3胁迫下植物细胞能够提升交替呼吸途径的容量,从而在一定程度上限制了细胞H2O2的积累,并由此缓解了细胞死亡的发生.

英文摘要：

By using BY-2 tobacco suspension cells, the effect of the alternative oxidase on cell death induced by AlCl3 stress was studied and the results showed that with the increase of the concentrations of AlCl3 （30, 50, 80, 100μmol/L）, the level of cell death and the capacity of the alternative respiratory path increased. AlCl3 stress also caused an increase of the content of hydrogen peroxide （H2O2）, Compared with the cells under AlCl3 stress, the AlCl3-stressed cells pretreated with a specific inhibitor of alternative oxidase had a higher level of cell death and H2O2 production. Exogenous application of H2O2 caused notable cell death in a dose-dependent manner. Application of the scavenger of H2O2 alleviated the cell death induced by AlCl3 and exogenous H2O2. These results suggest that the alternative oxidase could play a role in alleviating the cell death induced by AlCl3 stress and such a role of the alternative oxidase could relate to its ability to limit the H2O2 accumulation under AlCl3 stress.