中文摘要：

辐射敏感蛋白23具有核苷酸切除修复功能,在泛素蛋白酶体途径中起到重要作用。本研究利用PCR技术克隆了日本血吸虫辐射敏感蛋白23（Sj RAD23）编码的c DNA序列,成功获得Sj RAD23的基因序列,其ORF为1 053 bp。构建Sj RAD23基因重组表达质粒p ET28a（＋）-Sj RAD23,并在大肠杆菌BL21中成功诱导表达,重组蛋白在上清和沉淀中都有存在。利用免疫组化技术检测该蛋白在虫体的分布情况,该蛋白广泛分布在日本血吸虫虫体被膜。用重组蛋白免疫BALB/c小鼠后,免疫小鼠血清中检测到较高水平的特异性Ig G、Ig G1和Ig G2a。Western blotting分析显示重组蛋白能够被日本血吸虫成虫可溶性抗原免疫小鼠血清所识别。用重组蛋白r Sj RAD23免疫结果与206佐剂对照组比较,r Sj RAD23在BALB/c小鼠中诱导了35.94%减虫率,40.59%肝脏减卵率。结果表明Sj RAD23具有作为疫苗候选分子的潜力。

英文摘要：

Radiation sensitive protein 23（RAD23） is a nucleotide excision repair（NER） protein that plays an important role in Ubiquitin-proteasome pathway（UPP）. Schistosoma japonicum radiation sensitive protein23（Sj RAD23） c DNA sequences were amplified by PCR and cloned into p ET28a（＋） vector to construct recombinant expression plasmid p ET28a（＋）-Sj RAD23. The recombinant protein was expressed as both inclusion bodies and the supernatant in Escherichia coli BL21（DE3） cell. Immunofluorescence observation shows that Sj RAD23 was mainly distributed on the tegument surface of the worms. ELISA assay reveals that specific Ig G, Ig G1 and Ig G2 a antibodies could be detected in the sera of r Sj RAD23 immunized mice. Western blotting analysis shows that the recombinant Sj RAD23 could be recognized by serum specific to soluble adult worm antigen of S. japonicum. BALB/c mice vaccinated with r Sj RAD23 combined with 206 adjuvant revealed 35.94% worm reduction and 40.59% liver egg reduction when compared with that of the adjuvant control group.This study suggests that r Sj RAD23 has the potential as a vaccine against schistosome infection.