中文摘要：

目的研究锰对脑多巴胺神经细胞的毒性作用，重点观察α-突触核蛋白与细胞凋亡的改变。方法利用体外脑片培养模型，用不同浓度氯化锰（0，25，100，400μmol／L）处理脑片24h后，观察脑片中酪氨酸羟化酶（tyrosinehydroxylase，TH）阳性细胞改变，培养液中乳酸脱氢酶（lactatedehydrogenase，LDH）的释放量，细胞凋亡率以及α-突触核蛋白的表达。结果随着Mn处理浓度的增加，脑片神经细胞损伤逐渐加重。与对照组比较，锰处理脑片导致TH阳性细胞数明显减少，LDH释放量、细胞凋亡率以及α-突触核蛋白的表达均明显增加。结论锰对多巴胺神经细胞有毒性作用，并且锰可以通过诱导α-突触核蛋白过表达而造成神经细胞损伤。

英文摘要：

Objective The aim Of this study is to explore the toxic effects of manganese（Mn） on brain dopamine nerve ceils on brain slices,especially focus on the change of Mn-induced protein expression of alpha-synuclein and apoptosis. Methods Using cultured brain slice model in vitro,treated with different concentrations of Mn（0,25,100,400 μmol/L）for 24 h,detect the change of TH-positive cells, lactate dehydrogenase （LDH）release amount in culture medium;apoptosis rate and the protein expres- sion pattern of alpha-synuclein. Results With the increase of Mn concentration, nerve cell damage gradually aggravated in brain slice ; compared with control group, TH positive cells obviously reduced in Mn-treated brain slices, while the LDH release amount, apoptosis rate and alpha-synuclein expression level were all significantly increased. Conclusion The results suggested that Mn had toxic effect on dopaminergic neurons, which could damage neurocytes through inducing over-expression of alpha-synuclein.