中文摘要：

目的比较牙龈结合上皮（junctional epithelium，JE）与口腔龈上皮（oralepithelium，OE）细胞的生长附着特性，观察附着结构的形成过程，为牙周附着的形成提供实验依据。方法人JE和OE取自正畸或阻生原因拔除的牙周健康牙，采用酶消化法和无血清角质细胞培养液分离培养细胞。将5×10^8个／LJE细胞1ml接种到放置于24孔板的预处理5m×3mm×1mm牙骨质片上（每孔3片，共21片），于37℃5％CO2培养箱中复合培养，1～14d内取样本，透射电镜下观察附着结构的形成；同法以人OE细胞一牙骨质片复合培养作对照研究。结果JE细胞形态多样，排列散乱，CK19表达强阳性；OE细胞呈多边形或角形，“铺路石”状排列，仅少量OE细胞CK19阳性。JE细胞-牙骨质片复合培养1～3d，牙片上附着细’胞少，9d时接触牙片的细胞膜局部出现少量电子致密沉积物，11—14d出现复层生长，与牙面接触处可见半桥粒样结构；而OE细胞一牙骨质片复合培养1—3d牙骨质片上细胞较多，部分已伸展成扁平形，7d时细胞膜和牙面间出现大量颗粒状电子致密沉积物，9d时细胞呈复层生长，可见半桥粒样结构。结论人JE细胞是一种不同于OE细胞的未成熟、低分化细胞，在同样条件下体外培养的JE细胞在牙片上的生长和附着均较OE细胞慢，其对牙面的附着强度值得深入研究。

英文摘要：

Objective To compare the attachment and growth characteristics between human junctional epithelium（JE）and oral epithelium cells. Methods The healthy JE biopsies were derived from the human teeth extracted due to impaction or orthodontic purpose. Enzyme digestion was used to isolate JE cells, which were then cultured in DKGM. The co-culture model of JE cell-tooth slice was built up by adding 3 decalcification cementum slices （5 mm × 3 mm × 1 mm） into sterilized plate containing 1 ml of JE cells （5 × 10^8/L）, 21 slices all together,and incubated in an atmosphere containing 5% CO2 at 37 ℃ for 1-14 days. The attachment structrure was observed under transmission electron microscope, and the OE cells was used as control. Results The human JE cells were polymorphous in shape and CK19 posotive, while OE cells were consisted of equal and closely packed epithelial-like cells in a paving stone arrangement, and CK19 was only strained in a few cells. There were a few cells in JE-slice when co-cultured for 1-3 days, and electron dense plaques on the JE cell surface of the attached slice were observed at 9 days, and 2-3 layer of JE cells and hemidesmosome-like structure formed within 11-14 days. There were more OE cells within 1- 3 days, electron dense plaques appeared at 7 days, and stratified epithelium and hemidesmosome-like structure formed in OE-slice at 9 days. Conclusions The cultured JE cells were immature and lower differentiated epithelial cells which were different from OE cells. Under the same condition the growth and attachment of JE cells on the cementum slice surface were slower than that of OE cells. Their attachment strength needs further study.