中文摘要：

采用改进的CTAB法提取中国特有极度濒危植物猪血木（Euryodendron excelsum H．T．Chang）嫩叶总DNA，并对其进行RAPD分析，分别检测了模板DNA、引物、dNTP、Mg^2＋和Taq DNA聚合酶用量对反应结果的影响．筛选并建立了适合于猪血木RAPD扩增的反应体系：总体积25止，其中10×PCR缓冲液（500mmol／L KCl，100mmol／L Tris—HCl，110％Triton X-100，20mmol／L MgCl2）2．5μL，引物（1．5μmol／L）0．3μL，dNTPs（10mmol／L）0．5μL，模板2μL（25ng），Taq酶0．2μL（0．5U）．

英文摘要：

The DNA templates of Euryodendron excelsum H. T. Chang extracted from young leaf were amplified by RAPD. Effects of the content of DNA temlates, primers, dNTP, Mg^2＋ and Taq DNA polymerase on experimental results were tested and the optimal reaction system of RAPD for Euryodendron excelsum H.T. Chang was determined as follows：25 ng DNA template,2.0 mmol/L Mg^2＋ ,0.018 μmol/L primer,250 μmol/L dNTP,0.5 u Taq DNA polymerase in total 25 μL reaction volume.