中文摘要：

目的分析SLE患者调节性T细胞中微小RNA（miR）-326mRNA的表达水平与SLE临床特征之间的关联，探讨调节性T细胞中miR-326在SLE发病中的作用。方法收集52例SLE患者和20名健康对照者的资料，并采集抗凝静脉血20ml。磁珠分选法分选调节性T细胞，实时荧光定量PCR技术检测调节性T细胞中miR-326的表达水平。采用秩和检验及Spearman相关分析进行统计学分析。结果SLE组调节性T细胞中miR-326mRNA的表达水平[2．927（0，518，8．662）]高于健康对照组[0．921（0．345，1．879）]（Z=-3．756，P〈0．05）；新发SLE组及复发SLE组miR-326mRNA的表达水平分别为[8．878（5．922，12．466）]和[3．512（0．582，11．223）]，显著高于健康对照组（z=-2．135，Z=-4．928，P均〈0．05）。新发SLE组miR．326的表达水平高于稳定SLE组[1．178（0．508，3．211）]（Z=-4．928，P〈0．05）。在有浆膜腔积液的新发SLE患者的调节性T细胞中miR．326mRNA的表达水平[7．606（0．656，16．795）]高于无浆膜腔积液的患者[1．840（0．576，13．025）]（Z=4．263，P〈0．05）。新发SLE组患者调节性T细胞中miR．326mRNA表达水平与抗C1q抗体（仁0．729，P〈0．05）及CRP（rs=0．481，P〈0．05）水平呈正相关。结论miR．326mRNA在新发SLE患者调节性T细胞中显著升高，并与部分疾病活动指标相关，提示调节性T细胞中miR．326可能与早期SLE的疾病发生及活动相关。

英文摘要：

Objective To analyze the association of miR-326 mRNA expression level on regulatory T cells between clinical manifestations of patients with systemic lupus erythematosus （SLE）, in order to inves-tigate the role of miR-326 on Treg cells and pathogenesis as well as its association with disease activity of SLE. Methods Twenty milimeter anti-coagulated peripheral blood was obtained from 52 SLE patients and 20 healthy controls. Treg were purified by MACS from peripheral blood, in which the quantity of miR-326 and Ets-1 mRNA were assessed by real-time polymerase chain reaction （PCR）. Data were analyzed by using Mann- Whitney U test and Spearman correlation analysis. Results Compared with healthy controls [0.921 （0.345, 1.879）], the expression of miR-326 mRNA level was significantly higher in Treg from SLE patients [2.927 （0.518, 8.662） （Z=-3.756,P〈0.05）. The difference between new-onset SLE patients [8.878（5.922, 12.466）] and recurrence group [3.512（0.582, 11.223）] with healthy controls was significant （Z=-2.135, Z=-4.928, P〈0.05）. The expression level of miR-326 in new-onset SLE patients was higher than inactive SLE patients （Z=-4.928, P〈0.05）. Significant difference of the expression level of miR-326 mRNA was found between new-onset SLE patients with serous cavity effusion [7.606（0.656, 16.795）] and new-onset SLE patients without it [1.840（0.576, 13.025）] （Z=4.263, P〈0.05）. Our analysis showed that significant positive correlation was found between the expression of miR-326 mRNA in Treg with CRP （rs=0.481, P〈0.05） and anti-Clq antibody （rs=0.729, P〈0.05） from new-onset SLE patients. Conclusion The expression level of miR-326 is upregulated in Treg from SLE patients and is associated with disease active index, which suggests that miR-326 in Treg may participate the pathological process and disease activity in SLE.