中文摘要：

目的：探讨大鼠脂肪来源干细胞（adipose-derived stem cells,ADSCs）对放射后成纤维细胞（fibroblast,Fb）的影响。方法：体外原代培养SD大鼠ADSCs和真皮Fb,分别对两种细胞进行鉴定,Fb辐照后两种細胞建立共培养模型。将Fb分为3组,对其进行lncRNA和mRNA转录本高通量测序。结果：ADSCs高表达CD29和CD44,低表达CD31和CD45。大鼠Fb高表达波形蛋白。Fb单纯辐照和干細胞干预后转录水平有明显的差异。结论：ADSCs能使辐射后的Fb細胞lncRNA和mRNA转录水平明显的改变,提示多项生物学功能及信号通路在其中发挥作用。

英文摘要：

Objective：To discuss the effect of rat adipose derived stem cells（ADSCs） on fibroblasts（Fb） after radiation.Methods：We cultivated ADSCs and dermal Fb of SD rats in vitro,and two kinds of cells were identified.Then a co-culture model was established.Fb was divided into 3 groups,which carry on lncRNA and mRNA high throughput sequencing.Results：The high expression of CD29 and CD44 were identified in ADSCs,and the low expression of CD31 and CD45 in Fb.There was a significant difference in the level of transcripts between control group and Fb group of ADSCs Intervention after radiation.Conclusion：ADSCs can significantly alter the lncRNA and mRNA transcription levels of Fb cells after irradiation,suggesting that a number of biological functions and signaling pathways play an important role in the prcess.