中文摘要：

目的：观察血管生成素1（Ang-1）在小鼠卵巢组织中的表达,探讨其对小鼠卵泡发育、排卵以及黄体形成与退化的影响。方法：建立小鼠性成熟模型、马绒毛膜促性腺激素（eCG）诱导的卵泡发育模型、人绒毛膜促性腺激素（hCG）诱导的排卵模型以及黄体形成和退化模型,RT-PCR法检测不同时间Ang-1在不同模型的小鼠卵巢组织中的表达。结果：Ang-1 mRNA在小鼠出生后第10和20天表达较低;在出生后第25天,Ang-1mRNA的表达量升高,与第10天比较差异有统计学意义（P〈0.01）;之后Ang-1表达逐渐降低。在注射eCG后,Ang-1mRNA的表达量均高于0h组,在48h表达量达到最高值（P〈0.01）。注射hCG后3、5和9h,Ang-1mRNA表达量降低;而在hCG注射后0.5和7.0h,Ang-1的表达量较高;各组与0.5h组比较差异均有统计学意义（P〈0.01）。Ang-1mRNA在hCG注射后1～15d均有表达,且在注射后5～15d,Ang-1的表达逐渐升高,与1d组比较差异均有统计学意义（P〈0.05或P〈0.01）;在注射hCG后第15天,Ang-1的表达水平最高。结论：Ang-1可能参与了小鼠卵泡发育、排卵和黄体形成与退化的过程。

英文摘要：

Objective To detect the expression of angiopoietin-1（Ang-1） in mouse ovary tissue and to discuss the influence of Ang-1 on mouse follicular development,ovulation and formation and degradation of corpus luteum.Methods The sexual maturation model,follicular development induced by equine chorionic gonadotropin（eCG） model,ovulation induced by human chorionic gonadotropin（hCG） model and corps luteum formation and regression model were set up.The expressions of Ang-1 in the mouse ovary tissue at different time in different models were detected by RT-PCR.Results The Ang-1 mRNA expression levels were lower on the 10th and 20th day after birth,but it was increased on the 25th day after birth compared with the 10th day group（P〈0.01）;then it was gradually decreased.The Ang-1 mRNA expression was increased compared with 0 h group（P〈0.01） and it reached the peak level at 48 h after injection of eCG.At 3,5 and 9 h after injection of hCG,the Ang-1 mRNA expression was decreased;while at 0.5 and 7.0 h after injection of hCG,the Ang-1 mRNA expression levels were higher;there were significant differences between 0.5 h group and other groups（P〈0.01）.The Ang-1 mRNA expressed on the 1st day to the 15th day after injection of hCG,and on the 5 th day to the 15th day after injection of hCG,the Ang-1 expressions were increased gradually compared with 1 d group（P〈0.05 or P〈0.01）;and on the 15th day after injection of hCG,the Ang-1 expression level reached the peak.Conclusion Ang-1 may plays a role during the process of mouse follicular development,ovulation and corpus luteum formation and regression.