中文摘要：

[目的]探究独一味组培快繁技术体系,保护并合理利用独一味资源.[方法]选取独一味无菌苗的子叶、嫩芽和幼根为外植体,采用不同类型的培养基和不同种类的植物生长调节剂进行组培快繁研究.[结果]独一味幼叶、嫩芽和幼根均可诱导出愈伤组织,其中幼根的愈伤组织诱导率最高,达93.5％,出愈时间为7d,最适诱导培养基为MS ＋1.0mg/L2,4-D＋ 0.5 mg/L 6-BA＋ 0.5 mg/L NAA;丛生芽诱导的适宜培养基为MS＋ 1.0 mg/L 6-BA＋ 0.5 mg/L NAA,诱导率达88.8％;生根的适宜培养基为1/2 MS＋ 0.5 mg/L NAA,诱导率高达97.9％.[结论]利用组织培养技术能够实现独一味的快速繁殖,为独一味资源的可持续利用提供参考.

英文摘要：

[Objective] It is of significance to establish tissue culture system of Lamiophlomis rotata （Benth.） Kudo in order to rationally protect and utilize its resources.[Method] The cotyledons,tender shoots and tender roots of the aseptic seedling as explants were cultured in the media with different plant growth regulators to find out more reasonable medium formula for the induction of callus,cluster buds and rooting.[Results] The tender roots were the optimal explants with the highest callus induction rate （93.5%）and the shortest induction time （7 d）,and the best medium for callus induction was MS ＋ 1.0 mg/L 2,4-D ＋ 0.5 mg/L 6-BA ＋ 0.5 mg/L NAA; The optimal medium for cluster buds induction was MS ＋ 1.0 mg/L 6-BA ＋ 0.5 mg/L NAA,induction rate was 88.8% ; The optimal medium for rooting culture was 1/2 MS ＋0.5 mg/L NAA,in which the frequency roots reached 97.9%.[Conclusion] This effective approach for rapid propagation would better ensure the sustainable use of Lamiophlomis rotata（Benth.） Kudo.