中文摘要：

利用超高效液相色谱-飞行时间质谱联用技术（UPLC-TOF-MS）分析人参麦冬药对配伍后人参皂苷的变化,从化学成分层次阐释其配伍机制。采用Acquity BEH C18色谱柱,流动相为0.1%甲酸水溶液和乙腈溶液梯度洗脱,电喷雾电离离子源V模式检测,建立基于UPLC-TOF-MS的人参麦冬药对配伍的化学指纹图谱,数据分析采用MassLynx 4.1软件通过主成分分析法和正交偏最小二乘判别法分析药对配伍在合煎过程中人参皂苷的成分变化,找出差异变化显著的化学成分。结果表明：人参麦冬药对合煎液与合并液相比,8种皂苷成分发生显著变化,其中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1、丙二酸甲酰基人参皂苷Rb1的含量减少,而人参皂苷Rb3、丙二酸甲酰基人参皂苷Rb2、丙二酸甲酰基人参皂苷Rc、人参皂苷Rf的含量增加,说明麦冬与人参配伍改变了人参皂苷成分的含量,这可能是麦冬配伍人参作用的物质基础。

英文摘要：

An ultra performance liquid chromatography coupled with time-m-fight mass spectrometry （UPLC-TOF-MS） based on chemical profilingapproach was used to evaluate chemical constitution between co-decoction and mixed decoction of ginseng and radix ophiopogonis. Two different kinds of decoctions, namely ginseng and radix ophiopogonis co-decoction （water extract of mixed two herbs）, and ginseng and radix ophiopogonis mixed decoction （mixed water extract of each individual herbs） were prepared. The chromatography was performed using Acquity BEH C18 column, the mobile phase consisted of （A） water containing 0.1% formic acid and （B） acetonitrile, mass spectrometry was performed on an electrospray ionization source operating in V mode. Batches of these two kinds of decoction samples were subjected to UPLC-TOF-MS analysis, the datasets of tR-m/z pairs, ion intensities and sample codes were processed with supervised partial least squared discriminant analysis （OPLS-DA） to holistically compare with the difference of these two kinds of decoction samples. Results show that the content of ginsenoside Rgl, ginsenoside Re, ginse oside Rbl and malonylginsenoside Rbl are decreased, while the content of ginsenoside Rb3, malonylginsenoside Rb2, malonylginsenoside Rc and ginsenoside Rf are increased in samples of co-decoction of ginseng and radix ophiopogonis. The content of protopanaxatriol type ginsenoside decreased, while the content of protopanaxadiol type ginsenoside increased, which may be basis of chemical composition in combination of ginseng and radix ophiopogonis.