中文摘要：

研制能用于96孔酶标板快速检测的微型丝网印刷电极,并建立克仑特罗的电化学分析方法.通过间接竞争免疫反应与计时电流法相结合实现克仑特罗的残留量的定量测定.实验中考察并优化免疫反应与酶催化反应的条件.结果表明：在优化的条件下,克仑特罗标准溶液质量浓度在0.025～2.0 μg/L范围内呈现良好线性关系（r为0.999 2）.采用本实验方法检测猪肉和猪尿中克仑特罗的结果与商品化ELISA试剂盒一致.本方法检测限分别为0.18 μg/L和0.05 μg/L,比ELISA试剂盒检测限低一个数量级.

英文摘要：

An electrochemical sensing method for the detection of clenbuterol （CLB） was developed based on miniscreen printed electrodes and 96-well mierotiter plate.Clenbuterol residues were indirectly detected by combined use of indirect competitive immunoreaction and chronoamperometry.The immune reaction and enzymatic reaction conditions were investigated.Under the optimized conditions,the results showed that the electrochemical immunosensor produced a linear range of 0.025-2.0 μg/L with a correlation coefficient of 0.999 2.The analytical results of CLB residues in pork and swine urine samples were consistent with those obtained with an enzyme-linked immunosorbent assay （ELISA） plate.The detection limits for pork and swine urine samples were 0.18 μg/L and 0.05 μg/L,respectively,showing a decrease of one order of magnitude in comparison with those of ELISA.