中文摘要：

目的通过噬菌体展示技术筛选丹参-人参组分复方（丹参总酚酸、人参总皂苷、人参多糖）的作用靶点。方法经过T7噬菌体展示人肺癌c DNA文库的4轮淘选,获得阳性噬菌体克隆,随机挑选10个克隆进行DNA提取和测序。基因序列用Ex PASy网站的Translate tool查找开放阅读框（ORF）。选取代表性多肽用生物膜干涉技术检测亲和力。结果 4轮淘选后阳性噬菌体克隆得到高度富集,10个样品中7个有相同的DNA序列,长度为471 bp（序列1）,另外3个完全一致,长度为58 bp（序列2）。序列1得到4个ORFs,序列2未进行分析。丹参-人参组分复方和多肽His-MNTGRFGKTTSSPALTLGNFQKPL亲和力最高,KD=4.57×10^（-8）mol/L,人参多糖、丹参总酚酸和多肽亲和力分别为KD=7.23×10^（-8）mol/L、KD=7.66×10^（-8）mol/L,人参总皂苷与多肽没有典型的结合和解离效应。结论本研究获得了与丹参-人参组分复方高亲和力多肽,为丹参-人参组分复方肺癌靶向治疗研究提供依据。

英文摘要：

AIM To use phage display technique to screen targets for compound prescription of Salviae Miltiorrhizae Radix et Rhizoma and Ginseng Radix et Rhizoma（ total salvianolic acid,total ginsenoside,ginseng polysaccharides）. METHODS The positive phage clones were obtained with T7 select human lung tumor c DNA library after biopanning four times. Ten phage clones were randomly picked for DNA sequence analyses. Gene sequences were found open reading frames（ ORFs） with Ex PASy website Translate tool. The representative polypeptide was selected for the test of binding affinity by biolayer interferometrvy technolgy. RESULTS The positive phage clones were gradually enriched after biopanning; seven out of ten phage clones had the same DNA sequence with471 bp in length,whereas other three phage clones were identical with 58 bp in length. Four ORFs came from Sequence 1. Sequence 2 was not analyzed. There was high affinity between compound prescription and the polypeptide His-MNT GRFGKTTSSPALTLGNFQKPL,KD= 4. 57 ×10^（-8）mol/L. KDvalues of affinity of ginseng polysaccharides and total salvianolic acid were 7. 23 ×10^（-8）mol/L and KD= 7. 66 ×10^（-8）mol/L,respectively. However,there were not typical association and dissociation between total ginsenoside and the polypeptide. CONCLUSION The high affinity polypeptide obtained in this study combined compound prescription of Salviae Miltiorrhizae Radix et Rhizoma and Ginseng Radix et Rhizoma can be used in targeted therapy for lung cancer.