中文摘要：

目的：研究HEK-293T细胞中晶状体上皮源性生长因子（lens epithelium-derived growth factor,LEDGF）对富含酸性氨基酸的线粒体定位蛋白（Mitochondria-localized Glutamic Acid Rich Protein,MGARP）基因表达的调控作用。方法：将不同剂量的含有LEDGF基因的载体,转入HEK-293T细胞中,通过Western blot和启动子报告分析检测的方法,检测细胞内MGARP的表达水平。并采用LEDGF和Sp1共同转染的方法,来检测二者对MGARP转录活性的协同调控作用。结果：与空载体对照组相比,随着含有LEDGF基因载体剂量的增加,HEK-293T细胞内MGARP的表达也明显上调（P〈0.05）,同时共同转染LEDGF和Sp1后,细胞内MGARP的转录活性比单独过量表达LEDGF或者Sp1的转录活性明显增高（P〈0.05）。结论：在HEK-293T细胞中,LEDGF可以调控MGARP的表达,而且,LEDGF和Sp1对MGARP的调控具有协同作用。

英文摘要：

Objective： To study the role of lens epithelium-derived growth factor （LEDGF） in regulating Mitochondria-localized Glutamie Acid Rich Protein （ abbreviated as MGARP） in HEK-293T cells. Methods： The different dosages of LEDGF were transfected into the HEK-293T cells, which was followed by Western blot and promoter report to detect the expression of MGARP in HEK-293T cell. Furthermore, we co-tmnsfected Spl with LEDGF into cells to test their synergistic regulation to the transcriptional activity of MGARP promoter. Results： As the increase of LEDGF dosages, the expression of MGARP was significantly upregulated compared with the control（P〈0.05）, and after co-transfection of Sp I with LEDGF, the promoter activity of MGARP was remarkably enhanced and much higher than that transfected with either LEDGF or Spl individually （P〈0.05）. Conclusions： In HEK-293T cells, LEDGF can up-regulate the expression ofMGARP gene and such regulation can be synergistically enhanced by Sp1.