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Mapping of Microsatellite SW943 to Porcine Chromosome 12p11-(2/3p13) Using Primed in situ Synthesis and Somatic Cell Hybrid Panel
  • 分类：S[农业科学]
  • 作者机构：Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China
  • 相关基金：This research was supported by the National Key Projects of Basic Research and Development Plan (G2000016103), the National Natural Science Foundation of China(39970541), the National Outstanding Youth Science Foundation(3992507) and the Doctorate Foundation of the Minister of Education of China (1999004004).
作者： LIU Bang WANG Yong-qiang ZHANG Qing-de YU Mei ZHAO Shu-hong XIONG Tong-an LI Kui
关键词： PRIMER, in, situ, synthesis, , SOMATIC, cell, HYBRID, panel, , Microsatellites, , Physical, mapping, , Pigs
中文摘要：

The porcine microsatellite SW943 was regionally localized on 12p11-(2/3p13) by the two methods: the Primed in situ (PRINS) labelling on the pachytene bivalents of pigs using the Dig-11-dUTP as the report molecule and pig × rodent Somatic Cell Hybrid PaneI(SCHP) which contains 27 cell lines through PCR amplification. Advantages and disadvantages of the two methods for physical mapping of microsatellites were also discussed.

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