中文摘要：

鉴定得到嗜热四膜虫13个含有完整保守结构域的hsp70基因,对其中5个高度相似且无内含子的hsp70基因进行表达分析。在37、39和41℃热激条件下,实时荧光定量PCR结果表明,hsp70-2基因对热激响应最敏感。在四膜虫生长、饥饿和接合生殖这3种生理或发育状态下,Microarray结果显示,hsp70-4基因恒定且高表达;在热激条件下,hsp70-4基因的表达水平随着温度的升高而略微增加,证实hsp70-4基因为热休克相关蛋白hsc70基因;克隆的hsp70-4基因全长2208bp,开放阅读框长1959bp,编码653个氨基酸。Microarray结果提示,hsp70-3可能参与四膜虫饥饿早期（0～12h）的耐受和接合生殖后期（6～10h）的新大小核形成,老大核凋亡等事件;hsp70-5可能参与四膜虫饥饿晚期（12～15h）的耐受和接合生殖早期（0～6h）的小核减数分裂、小核交换和原核（pronuclear）融合事件。Blast2GO分析表明,与hsp70-3和hsp70-5共表达的基因分别参与不同的生物学过程,进一步反映了hsp70-3和hsp70-5这两个基因在功能上是存在差异的。

英文摘要：

Thirteen hsp70 genes with complete conserved domains were identified in Tetrahymena thermophila,and expression of five similar and non-intron hsp70 genes were analyzed.Under heat shock conditions of 37,39 and 41oC,hsp70-2 mRNA had the highest relative expression level,suggesting it is closely related to heat shock.The basal level of constitutive T.thermophila hsp70-4 gene was high during 20 physiological/developmental stages of growth,starvation and conjugation,and it changed little upon exposure to heat shock：evidence that hsp70-4 is an hsc70 gene.The hsp70-4 cDNA is 2 208 bp long,and contains an open reading frame of 1 959 bp encoding 635 amino acids.Microarray data indicated that T.thermophila hsp70-3 gene probably participated in early starvation（0-12 h） stress and late conjugation（6-10 h） events,such as new macronuclear and micronuclear anlagen formation and old macronuclear elimination.However,hsp70-5 gene possibly participates in late starvation（12-15 h） stress and early conjugation（0-6 h） events such as micronuclear meiosis,micronuclear exchange and pronuclear fusion.Blast2GO indicated that they participated in dissimilar biological processes,suggesting hsp70-3 and hsp70-5 perform different functions.