中文摘要：

目的研究一水草酸钙晶体（calcium oxalate monohydrate,COM）引起的不同表型巨噬细胞的炎症反应。方法在大鼠草酸钙结石模型中研究肾脏巨噬细胞的浸润情况,明确是否存在肾脏炎症。体外分离培养小鼠骨髓源巨噬细胞,流式细胞仪鉴定其细胞表面标志。将巨噬细胞体外分化为M1和M2,Real-time PCR鉴定细胞标志。Real-time PCR检测COM及损伤标志分子ATP对巨噬细胞IL-1β表达的影响,ELISA法检测其IL-1β的分泌。结果在大鼠草酸钙结石模型肾脏切片中,可见大量巨噬细胞浸润,显著多于对照大鼠。体外分离小鼠巨噬细胞,流式细胞仪鉴定表面标志符合巨噬细胞表型,Real-time PCR鉴定M1和M2的标志也符合其相应表型。M1高表达IL-1β、CCL2,低表达MR、ARG;M2高表达MR、ARG,低表达IL-1β、CCL2。COM及其损伤分子ATP均可使M1高表达IL-1β。结论在草酸钙大鼠模型中,巨噬细胞浸润明显,COM和ATP可加重巨噬细胞炎症,并可加重M1的促炎症作用。

英文摘要：

Objective To examine the calcium oxalate monohydrate（COM）-induced inflammation response of macrophages with different phenotypes.Methods The infiltration of macrophages was evaluated in the rat model of calcium oxalate stone to confirm the inflammation in the kidney.The mouse bone marrow derived macrophages were isolated in vitro and identified by flow cytometry.The macrophages were allowed to differentiate into M1 and M2 in vitro and their markers identified by realtime PCR.The effect of COM and ATP on the expression of IL-1βwas detected by real-time PCR and the secretion of IL-1β by ELISA.Results A greater number of macrophages were found infiltrating the kidney tissues in the rat model of calcium oxalate stone.Flow cytometry confirmed that the surface markers of cells isolated from the mouse were consistent with those of macrophages.Real-time PCR showed that the markers of M1 and M2 were in accordance with the phenotypes of M1 and M2.M1over-expressed IL-1β and CCL2,and down-expressed MR and ARG.The expression levels of the four molecules in M2 were on the contrary to those in M1.COM and ATP could induce the secretion of IL-1β by M1.Conclusion In the rat model of calcium oxalate stone,the macrophages infiltrate the kidney tissues obviously.COM and ATP exacerbate the inflammation response of macrophages and the M1-induced inflammation.