有 Taq DNA 聚合酶(Taq ) 的胶体的黄金的相互作用在这研究被调查。Taq 黄金结合被把酶加到胶体的金答案,由紫外力的光谱学证实了形成 X 光检查光电子光谱学,和光子十字关联光谱学大小。conjugate 被传播电子显微镜学进一步描绘。Taq 黄金结合粒子,这被发现仍然球形、分散得好。Taq 的 bioactivity 上的金 nanoparticles 的影响被分析聚合酶链反应扩大的收益学习。结果显示 Taq 的酶的活动在和胶体的金牌的相互作用以后减少了。
The interaction of colloidal gold with Taq DNA polymerase (Taq) was investigated in this study. Taq-gold conjugate was formed by adding the enzyme to the colloidal gold solution, as evidenced by UV-Vis spectroscopy, X-ray photoelectron spectroscopy, and photon cross correlation spectroscopy measurements. The conjugate was further characterized by transmission electron microscopy. It was found that the Taq-gold conjugate particles were still spherical and well-dispersed. The influence of gold nanoparticles on the bioactivity of Taq was studied by analyzing the yield of the polymerase chain reaction amplification. Results indicated that the enzymatic activity of Taq decreased after interaction with the colloidal gold.