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Monoclonal neutralizing antibodies against EV71 screened from mice immunized with yeast-produced virus-like particles
  • ISSN号:1674-0769
  • 期刊名称:《中国病毒学:英文版》
  • 分类:R392[医药卫生—免疫学;医药卫生—基础医学]
  • 作者机构:[1]State Key Laboratory of Virology, College of Life Sciences, Wuhan University
  • 相关基金:supported by the grant from National Natural Science Foundation of China (No.31070147)
中文摘要:

Periodic outbreaks of hand, foot and mouth disease(HFMD) occur in children under 5 years old, and can cause death in some cases. The C4 strain of enterovirus 71(EV71) is the main pathogen that causes HFMD in China. Although no drugs against EV71 are available, some studies have shown that candidate vaccines or viral capsid proteins can produce anti-EV71 immunity. In this study, female BABL/c mice(6–8 weeks old) were immunized with virus-like particles(VLPs) of EV71 produced in yeast to screen for anti-EV71 antibodies. Two hybridomas that could produce neutralizing antibodies against EV71 were obtained. Both neutralizing m Abs(D4 and G12) were confirmed to bind the VP1 capsid protein of EV71, and could protect 】 95% cells from 100 TCID50 EV71 infection at 25 μg/m L solution(lowest concentration). Those two neutralizing m Abs identified in the study may be promising candidates in development for m Abs to treat EV71 infection, and utilized as suitable reagents for use in diagnostic tests and biological studies.

英文摘要:

Periodic outbreaks of hand, foot and mouth disease(HFMD) occur in children under 5 years old, and can cause death in some cases. The C4 strain of enterovirus 71(EV71) is the main pathogen that causes HFMD in China. Although no drugs against EV71 are available, some studies have shown that candidate vaccines or viral capsid proteins can produce anti-EV71 immunity. In this study, female BABL/c mice(6–8 weeks old) were immunized with virus-like particles(VLPs) of EV71 produced in yeast to screen for anti-EV71 antibodies. Two hybridomas that could produce neutralizing antibodies against EV71 were obtained. Both neutralizing m Abs(D4 and G12) were confirmed to bind the VP1 capsid protein of EV71, and could protect > 95% cells from 100 TCID50 EV71 infection at 25 μg/m L solution(lowest concentration). Those two neutralizing m Abs identified in the study may be promising candidates in development for m Abs to treat EV71 infection, and utilized as suitable reagents for use in diagnostic tests and biological studies.

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期刊信息
  • 《中国病毒学:英文版》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院武汉病毒研究所 中国微生物学会
  • 主编:陈新文
  • 地址:湖北省武汉市武昌区小洪山中区44号
  • 邮编:430071
  • 邮箱:info@virosin.org
  • 电话:027-87199157
  • 国际标准刊号:ISSN:1674-0769
  • 国内统一刊号:ISSN:42-1760/Q
  • 邮发代号:38-351
  • 获奖情况:
  • 湖北省第1、2、3、4、5、6届优秀期刊,中国科学技术学会 优秀国际科技期刊,2012年中国国际影响力优秀学术期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),波兰哥白尼索引,荷兰医学文摘,美国生物医学检索系统,美国剑桥科学文摘,美国生物科学数据库,中国中国科技核心期刊
  • 被引量:208