欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的探讨应激所致肠功能紊乱大鼠模型建立和评价的方法。方法 SD大鼠随机分为肠功能紊乱模型组和正常组,模型组采用慢性束缚＋过度疲劳＋饮食失调法造模,3周后取血,用酶联免疫法（ELISA）试剂盒在血清测GAS、IL-2,在血浆中测ACTH、MTL、SS。结果模型组大鼠3周后与正常组相比,外观行为发生明显变化,体重和肝脾重量显著降低,生物标志物水平降低。结论通过慢性束缚＋过度疲劳＋饮食失调法可以建立稳定的应激肠功能紊乱大鼠模型,GAS、IL-2、ACTH、MTL、SS等内源性物质可作为模型的评价指标。
英文摘要：
Objective To explore the method of establishment of a rat model of stress-induced intestinal dysfunction and the method of its evaluation. Methods Sprague-Dawley rats were randomly assigned to two groups： the normal group and the model group. The model group was developed by combination of chronic constraint,excess fatigue and eating disorders for three weeks. At end of the third week,the serum GAS,IL-2,and plasma MTL,SS and ACTH were measured using an ELISA kit. Results Compared with the normal group,the model group had many distinct changes of appearance and behavior, weight loss, decrease of liver and spleen weight, and apparent changes of many biomarkers.Conclusions Stable rat model of stress-induced intestinal dysfunction can be successfully established by combination of chronic constraint,excess fatigue and eating disorders in SD rats,and the biomarkers such as GAS,IL-2,ACTH,MTL,SS can be used to evaluate this model.
同期刊论文项目
同项目期刊论文
Analysis of gene expression changes, caused by exposure to nitrite, in metabolic and antioxidant enz
Differences in growth, pigment composition and photosynthetic rates of two phenotypes Microcystis ae
Photosynthetic changes of flag leaves during senescence stage in super high-yield hybrid rice LYPJ g
Cloning, purification and biochemical properties of a thermostable pectinase from Bacillus haloduran
Synthesis and evaluation of a novel beta-cyclodextrin derivative for oral insulin delivery and absor
The complete mitochondrial genome of the hybrid of Pelteobagrus fulvidraco (female symbol) x Pelteob
TPGS2k/PLGA nanoparticles for overcoming multidrug resistance by interfering mitochondria of human a
Involvement of Relish gene from Macrobrachium rosenbergii in the expression of anti-microbial peptid
Efficient glycosylation of puerarin by an organic solvent-tolerant strain of Lysinibacillus fusiform
Resveratrol prevents cadmium activation of Erk1/2 and JNK pathways from neuronal cell death via prot
A novel vertebrates Toll-like receptor counterpart regulating the anti-microbial peptides expression
Identification, characterization, and functional studies of a Pelle gene in the Chinese mitten crab,
Inducible HSP70 antagonizes IL-1beta cytocidal effects through inhibiting NF-kB activation via desta
The complete mitochondrial genome of Pauropus longiramus (Myriapoda: Pauropoda): implications on ear
Molecular cloning of manganese superoxide dismutase gene in the cladoceran Daphnia magna: effects of
Inhibition of inflammatory mediators contributes to the anti-inflammatory activity of KYKZL-1 via MA
Molecular structure, expression pattern and functional characterisation of APRIL in an aquatic mamma
Growth inhibitory effect of KYKZL-1 on Hep G2 cells via inhibition of AA metabolites and caspase-3 p
Physiological and growth characteristics of Ginkgo biloba L. exposed to open ？eld and shade enclosur
Development and characterization of 42 novel polymorphic microsatellite markers for Odontobutis pota
Development of 39 novel polymorphic microsatellite markers for the giant mottled eel Anguilla marmor
Development and validation of RP-HPLC method for the simultaneous quantification of seven flavonoids
Liquid laccase production by Phomopsis iquidambari B3 accelerated phenolic acids degradation in long
Differences in long-term impacts of un-ionized ammonia on life-history traits of three species of Da
Morphological, physiological and biochemical responses of biofuel plant Euphorbia lathyris to salt s
Baicalein reduces lipopolysaccharide-induced in？ammation via suppressing JAK/STAT sactivation and RO
Molecular characterization, expression and binding activity of the cytokines TNFSF14 and its recepto
Characterization of polysaccharides with marked inhibitory effect on lipid accumulation in Pleurotus
Copper/zinc superoxide dismutase from the Cladoceran Daphnia magna: molecular cloning and expression
Sensitive and reliable multianalyte quantitation of herbal medicine in rat plasma using dynamic trig
Deep sequencing of small RNA repertoires in mice reveals metabolic disorders-associated hepatic miRN
Cloning and characterization of a clip domain serine protease and its homolog (masquerade) from Erio
Does microcystin disrupt the induced effect of Daphnia kairomone on colony formation in Scenedesmus?
Laccase production by Phomopsis liquidambari B3 cultured with food waste and wheat straw as the main
Metabolic and antioxidant responses in juveniles of Cherax quandricarinatus under actute cadmium str
The superoxide dismutase from red claw crayfish, Cherax quadricarinatus: molecular cloning and chara
Tunicamycin inhibits PDGF-BB-induced proliferation and migration of vascular smooth muscle cells thr
Cloning and characterization of two different L-type lectin genes from the Chinese mitten crab Erioc
期刊信息
位置：