中文摘要：

研究中国地质大学（武汉）校区3个池塘7个样点浮游生物群落的优势种组成和DNA指纹多态性.利用15种随机引物分别扩增,其中7种引物获得多态性片段.电泳共得到47条带,谱带平均多态率为96.4%.根据Nei＇s遗传距离矩阵构建3个池塘7个样点的遗传关系聚类树.通过优势种群组成聚类分析比较探讨浮游生物优势物种组成和DNA指纹遗传关系聚类树结构的关系.结果表明,同一池塘样点的优势种组成和DNA指纹遗传多样性相似性较高;西区池塘样点的优势种组成和遗传关系最近,其次是东区池塘的3个样点,差别最大的是北区池塘的两个样点.其原因可能是生境的隔离和差异.提示尽管池塘浮游生物优势种组成与DNA指纹结构表示的遗传关系会存在较小差异,但是分析结果基本一致.因此,利用DNA指纹技术研究池塘浮游生物生态学是可行的.

英文摘要：

Seven plankton community samples were collected from three ponds in the campus of China University of Geosciences in Wuhan,Hubei,China.All the samples were analyzed with RAPD fingerprinting polymorphism and the dominant species of plankton communities were identified.15 random primers were amplified and 7 primers obtained RAPD polymorphic fragments.47 bands were obtained after the electrophoresis and average band polymorphism rate was 96.4%.The genetic relation trees of the seven points in the three ponds were constructed according to the matrix of Nei＇s genetic distances.Relations between cluster trees of DNA fingerprinting topological structure and dominant species of plankton communities in the three ponds were discussed.The results showed that each pool had the similar plankton dominant species community and genetic relationship.The dominant plankton species community and genetic relations from the sampling sites in the west pond were most similar,and followed by the three sites in the east pond were followed.Those from the two sites in the north pond were most different.The reason might be that their living environments were different.Genetic similarity results based on DNA fingerprinting topological structure were in accord with the clustering results of dominant plankton species community except a small difference.Therefore,DNA fingerprinting technique is feasible to study the plankton ecology of ponds.