中文摘要：

目的：用转化生长因子（TGF-β1）诱导人肾小管上皮细胞（HK2）转分化，观察丹酚酸B（SA-B）对HK-2细胞转分化过程的干预作用并初步探讨其机制。方法：TGF-β1（5ng·mL^-1）刺激HK-2细胞，用丹酚酸B（SA—B）（10gmol·L^-1）干预；用免疫荧光法检测各组HK-2细胞黏着斑激酶（FAK）、整合素β1（β1-integrin）的表达。结果：TGF-β1成功诱导HK2转分化；相比空白对照组，SA-B可抑制HK-2转分化，且SAB可有效降低HK-2x细胞FAK及β1-integrin蛋白的表达（P均小于0．05）。结论：SA-B可有效调节人肾小管上皮细胞转分化过程，其机制可能与其能调控HK2细胞FAK及β1-integrin蛋白的表达有关。

英文摘要：

OBJECTIVE Using transforming growth factor（TGF-β1 ） to induce trans differentiation （EMT） of the tubular epithelial cell of HK-2 to myofibroblast so as to explore the possible mechanism of molecular signal transduction during the EMT process and explore the effect of salvianolic acid B （SA-B） on the transition of tubular epitheliai cell to myofibroblast. METHODS HK-2 were stimulated with TGF-β1, and then were handled with SA-B by using immunohistochemistry, the expression of FAK and Integrin-β1 was observed. RESULTS TGF-β1 induced the transdifferentiation of the tubular epithelial call to my ofibroblast successfully. Compared with the TGF-β1 group, the expression of FAK and integrin-β1 decreased in the SA B group（P〈0. 05）. CONCLUSION SA-B has effects on the EMT process of HK-2 through regulating the expression of FAK and β-integrin.