中文摘要：

Ran是细胞内的一种具有GTP酶活性的功能蛋白，可以调节染色体稳定性、细胞核组建以及核质运输等多种细胞进程．Ran结合蛋白1（Ran—binding protein1，RbplP）是Ran的必要调控因子，促进Ran—GTP水解为Ran—GDP．本研究从嗜热四膜虫大核基因组中鉴定出1个保守的Ran结合蛋白基因RBPl（TTHERM_00158040，http：／／www．ciliate．org）．实时荧光定量PCR表明，RBPl在四膜虫。养生长和有性生殖过程中都有表达，且在有性生殖过程中表达水平提高．免疫荧光定位表明，在营养生长期RbplP定位于细胞质中．过表达RBPl或敲减RBPl后，细胞生长速率下降，大核的无丝分裂异常，细胞分裂末期产生了无大核的异常细胞，同时过表达RBPl导致了多小核的产生．结果表明，RbplP影响四膜虫细胞核的分裂进程，它的正常表达对细胞增殖过程起到重要的调节作用．

英文摘要：

Ran regulates a variety of cellular processes via its intrinsic GTPase activity including chromosome stability, formation of the nucleus and nucleocytoplasmic transport. Ran-binding protein 1 （Rbplp） facilitates the activation of Ran GTPase as a necessary regulator in the Ran GTPase cycle. We identified an evolutionary conservative Ran-binding protein 1 gene from Tetrahymena thermophila termed RBP1 （TTHERM 00158040, http：//www, ciliate, org） which expressed in growing, starvation and conjugation stage. HA-tagged Rbplp was localized in the cytoplasm during vegetative growing stage. Over-expression or suppressed expression of Rbplp caused abnormal macronuclear amitosis or multi- micronuclear cells. These results that Rbplp affects of its normal expression in Tetrahymena thermophila the nuclear division suggested the important function