中文摘要：

目的：阐明神经钙黏蛋白（N—cadherin）在神经嵴细胞分层中的作用。方法：利用原位杂交鉴定N—cadherln在神经管上的表达。转染过表达N—cadherin的野生型N—cadherln（wild—typeN—cadherin，wt—N—cad）或阻断N—cadherin表达的结构域缺失型N—cadherin（dominant—negativeN—cadherin，dn—N—cad），利用免疫组化方法检测头部神经嵴细胞的迁移变化。结果：无论是过表达N—cadherin还是下调N—cadherin表达，头部神经嵴细胞迁移都受到了影响。结论：头部神经嵴细胞的分层和迁移依赖于神经管上N—cadherin的表达。

英文摘要：

AIM： To investigate the role of N - cadberin in the delamination of neural crest cells. METHODS： The normal expression of N - eadherin in neural tube was identified using in situ hybridization. The cells with N - eadherin over expression were obtained by transfection of wild -type N -cadherin （ wt - N -cadherin） , and the cells with N -cadber- in silencing expression were obtained by transfeetion of dominant - negative N - cadherin （ dn - N - eadherin）. The migra- tion of cranial neural crest cells was determined by the technique of immunohistochemistry. RESULTS： Either overexpres- sion or down - regulation of N - eadherin significantly affected the migration of cranial neural crest cells. CONCLUSION： Delamination and migration of the cranial neural crest cells rely on the relative N - eadherin expression in the neural tube during ncurulation.