在全部的 saponin 部分(TSF ) 之中评估化学侧面和细胞毒素的效果的目的, 25% 乙醇部分(25EF ) , 50% 乙醇部分(50EF ) ,和 85% 乙醇部分(85EF ) 从人参属 notoginseng 的叶子由 macroporous 树脂准备了。方法 13 主要 saponins 的同时的决心,以及化学药品 saponin 介绍不同极性的部分,被 HPLC 爸爸和 LC-ESI-MSn 分析做。细胞毒素的效果对 KP4 被决定房间(人的胰腺的癌症) , NCI-H727 房间(人的肺癌症) , HepG2 房间(人的 hepatocellular 癌症) ,和 SGC-7901 房间(人的胃的腺癌) 。结果化学药品分析显示 85EF 拥有了最丰富的细胞毒素的 protopanaxadiol saponins,包括标记 saponins F2, 20 (R)-Rg3, 20 (S)-Rg3, 和 Rh2。MTT 试金证明 85EF 也在四部分之中有最强壮的细胞毒素的效果。25EF 没显示出 anti-proliferative 效果,当 50EF 和 TSF 展出了弱 anti-proliferative 活动时。从资源的全面利用的方面的结论, 85EF,与低极性 PPD 组 saponins 充实,为进一步的 anticancer 研究是 anticancer saponins,和有希望的植物的准备的新其他的来源。
AIM: To evaluate the chemical profiles and cytotoxic effects among the total saponin fraction (TSF), 25% ethanol fraction (25EF), 50% ethanol fraction (50EF), and 85% ethanol fraction (85EF) prepared by macroporous resin from the leaves of Panax notoginseng. METHOD: The simultaneous determination of thirteen main saponins, as well as the chemical profiles of saponin fractions of different polarity, was made by HPLC-DAD and LC-ESI-MSn analysis. The cytotoxic effects were determined against KP4 cells (human pancreatic cancer), NCI-H727 ceils (human lung cancer), HepG2 cells (human hepatocellular cancer), and SGC-7901 cells (human gastric adenocarcinoma). RESULTS: Chemical analysis indicated that 85EF possessed the most abundant cytotoxic protopanaxadiol saponins, including the marker saponins F2, 20(R)-Rg3, 20(S)-Rg3, and Rh2. The MTT assay showed that 85EF also had the strongest cytotoxic effects among the four fractions. 25EF showed no anti-proliferative effects, while 50EF and TSF exhibited weak anti-proliferative activity. CONCLUSION: From the aspect of comprehensive utilization of resources, 85EF, enriched with low polarity PPD group saponins, is a new alternative source of anticancer saponins, and a promising botanical preparation for further anticancer studies.