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Chemical profiles and anticancer effects of saponin fractions of different polarity from the leaves of Panax notoginseng
  • ISSN号:2095-6975
  • 期刊名称:《中国天然药物:英文版》
  • 时间:0
  • 分类:R284[医药卫生—中药学;医药卫生—中医学] R285[医药卫生—中药学;医药卫生—中医学]
  • 作者机构:[1]Department of Pharmaceutical Analysis and Metabolomics,Jiangsu Branch of China Academy of Chinese Medical Science,Nanjing 210028,China, [2]State Laboratory of Modern Chinese Medicines,China Pharmaceutical University,Nanjing 210009,China, [3]Nanjing Normal University Center for Analysis and Testing,Nanjing Normal University,Nanjing 210009,China, [4]Jiangsu Center for New Drug Screening & National New Drug Screening Laboratory,China Pharmaceutical University,Nanjing 210009,China
  • 相关基金:This project was supported by the National Natural Science Foundation of China (Nos. 81274018, 81274068) and the Project funded by Jiangsu Branch of China Academy of Chinese Medical Science (JSBY 1306).
中文摘要:

在全部的 saponin 部分(TSF ) 之中评估化学侧面和细胞毒素的效果的目的, 25% 乙醇部分(25EF ) , 50% 乙醇部分(50EF ) ,和 85% 乙醇部分(85EF ) 从人参属 notoginseng 的叶子由 macroporous 树脂准备了。方法 13 主要 saponins 的同时的决心,以及化学药品 saponin 介绍不同极性的部分,被 HPLC 爸爸和 LC-ESI-MSn 分析做。细胞毒素的效果对 KP4 被决定房间(人的胰腺的癌症) , NCI-H727 房间(人的肺癌症) , HepG2 房间(人的 hepatocellular 癌症) ,和 SGC-7901 房间(人的胃的腺癌) 。结果化学药品分析显示 85EF 拥有了最丰富的细胞毒素的 protopanaxadiol saponins,包括标记 saponins F2, 20 (R)-Rg3, 20 (S)-Rg3, 和 Rh2。MTT 试金证明 85EF 也在四部分之中有最强壮的细胞毒素的效果。25EF 没显示出 anti-proliferative 效果,当 50EF 和 TSF 展出了弱 anti-proliferative 活动时。从资源的全面利用的方面的结论, 85EF,与低极性 PPD 组 saponins 充实,为进一步的 anticancer 研究是 anticancer saponins,和有希望的植物的准备的新其他的来源。

英文摘要:

AIM: To evaluate the chemical profiles and cytotoxic effects among the total saponin fraction (TSF), 25% ethanol fraction (25EF), 50% ethanol fraction (50EF), and 85% ethanol fraction (85EF) prepared by macroporous resin from the leaves of Panax notoginseng. METHOD: The simultaneous determination of thirteen main saponins, as well as the chemical profiles of saponin fractions of different polarity, was made by HPLC-DAD and LC-ESI-MSn analysis. The cytotoxic effects were determined against KP4 cells (human pancreatic cancer), NCI-H727 ceils (human lung cancer), HepG2 cells (human hepatocellular cancer), and SGC-7901 cells (human gastric adenocarcinoma). RESULTS: Chemical analysis indicated that 85EF possessed the most abundant cytotoxic protopanaxadiol saponins, including the marker saponins F2, 20(R)-Rg3, 20(S)-Rg3, and Rh2. The MTT assay showed that 85EF also had the strongest cytotoxic effects among the four fractions. 25EF showed no anti-proliferative effects, while 50EF and TSF exhibited weak anti-proliferative activity. CONCLUSION: From the aspect of comprehensive utilization of resources, 85EF, enriched with low polarity PPD group saponins, is a new alternative source of anticancer saponins, and a promising botanical preparation for further anticancer studies.

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期刊信息
  • 《中国天然药物:英文版》
  • 北大核心期刊(2008版)
  • 主管单位:中华人民共和国教育部
  • 主办单位:中国药科大学 中国药学会
  • 主编:吴晓明
  • 地址:南京童家巷24号中国药科大学2号信箱
  • 邮编:210009
  • 邮箱:cpucjnm@163.com
  • 电话:025-83271565 83271568
  • 国际标准刊号:ISSN:2095-6975
  • 国内统一刊号:ISSN:32-1845/R
  • 邮发代号:28-306
  • 获奖情况:
  • 国内外数据库收录:
  • 美国国际药学文摘,美国化学文摘(网络版),波兰哥白尼索引,美国生物医学检索系统,美国科学引文索引(扩展库),美国生物科学数据库,中国中国科技核心期刊,中国北大核心期刊(2008版)
  • 被引量:6564