中文摘要：

从猴头菇（Hericium erinaceus）子实体中，经过除杂、粉碎、热水浸提、醇沉处理后，得到了猴头菇杂聚糖粗提物，并运用 DEAE-cellulose column 进行柱层析分离纯化，得到猴头菇寡聚糖（HEO-A）．通过水解，单糖分析，扫描电镜技术（SEM ），高效凝胶渗透色谱（HPGPC），红外光谱技术（IR ）和核磁共振技术（NMR）对猴头菇寡聚糖进行了初步的结构分析．结果显示，猴头菇寡聚糖由木糖和葡萄糖两种单糖构成，均重分子量为1877 D ．在体外化学模拟条件下，研究了不同浓度的猴头菇寡聚糖对羟基自由基（·OH）、1，1-二苯基-2-苦苯肼自由基（DP-PH ·）及ABTS 自由基的清除作用．对DPPH ·自由基的清除能力，其IC50值为12．5 mg/mL ；对·OH自由基的清除能力，当 HEO-A 浓度达到5 mg/mL 时，其清除率可达到最大（37．51％）；对ABTS 自由基的能力，其IC50值为0．93 mg/mL ，且当 HEO-A 的浓度为1．5 mg/mL时，清除率即可达95．44％．这些结果说明猴头菇寡聚糖（HEO-A）具有显著的抗氧化功能，可以作为一种理想的天然抗氧化剂资源．

英文摘要：

A oligosaccharide was extracted from the fruiting bodies of Hericium erinaceus with water and then successively purified by DEAE-cellulose 52 column chromatography ,yielding one major oligosac-charide fractions ：HEO-A .Structural features of HEO-A were investigated by a combination of total hydrolysis ,high-performance gel permeation chromatography analysis （HPGPC） ,scanning electron mi-croscope （SEM） ,infrared （IR） spectra and nuclear magnetic resonance （NMR） spectroscopy studies . The results showed that the average molecular sizes of HEO-A were approximately 1877 D and was mainly composed of xylose and glucose .The antioxidant activity analyses revealed that HEO-A pos-sessed considerable antioxidant activity .The IC50 value of the DPPH · radical scavenging activity is 12 .5 mg/mL .The · OH radical scavenging ability reaches the maximum （37 .51% ） when the concentration of HEO-A reached 5mg/mL .The IC50 value of the ABTS radical scavenging activity is 0 .93 mg/mL , and w hen the concentration of HEO-A is only 1 .5 mg/mL ,removal rate is up to 95 .44% .Accordingly , Hericium erinaceus might serve as an effective antioxidative healthcare food and source of natural an-tioxidants .