中文摘要：

为了与改进抗病性，辣椒 annuum 的 AP1 基因和hygromycin抵抗基因( HPT )获得没有标记的转基因的米饭，分别地，被克隆进二进制向量 pSB130 的二个分开的 T-DNA 区域并且介绍了进我从二个精英装饰用的梨树大米变化， Guangling Xiangjing 和 Wuxiangjing 9 的不成熟的种子导出的电话，由 调停Agrobacterium 的转变调停了。包含 AP1 基因和标记基因的许多 cotransgenic 米饭线被改革，在转基因的米饭植物的两 transgenes 的集成被 PCR 或南部的弄污技术证实。几可选的没有标记的转基因的米饭植物随后从 cotransformants 的子孙被获得，并且由 PCR 和南部的弄污分析证实了。这些转基因的大米线在这块地里被测试，他们到疾病的抵抗小心地被调查，结果在接种以后显示出那抵抗到细菌的老家或鞘选择的转基因的线的老家被改进什么时候与那些相比野生型。

英文摘要：

In order to obtain marker-free transgenic rice with improved disease resistance, the AP1 gene of Capsicum annuum and hygromycin-resistance gene （HPT） were cloned into the two separate T-DNA regions of the binary vector pSB130, respectively, and introduced into the calli derived from the immature seeds of two elite japonica rice varieties, Guangling Xiangjing and Wuxiangjing 9, mediated by Agrobacterium-mediated transformation. Many cotransgenic rice lines containing both the AP1 gene and the marker gene were regenerated and the integration of both transgenes in the transgenic rice plants was confirmed by either PCR or Southern blotting technique. Several selectable marker-free transgenic rice plants were subsequently obtained from the progeny of the cotransformants, and confirmed by both PCR and Southern blotting analysis. These transgenic rice lines were tested in the field and their resistance to disease was carefully investigated, the results showed that after inoculation the resistance to either bacterial blight or sheath blight of the selected transgenic lines was improved when compared with those of wild type.