将中性盐NaCl、Na_2SO_4和碱性盐Na_2CO_3、Na HCO_3按不同比例混合,模拟6种不同p H的盐胁迫对大麻幼苗进行处理.通过形态生长指标及生理指标的测定,研究混合盐胁迫对大麻幼苗生长的影响.研究结果表明,株高、地上部干重、根长随p H的升高显著低于对照,而根冠比、地上部含水量显著高于对照;SOD活性、丙二醛含量均大幅度上升,可溶性糖含量呈先上升后下降的趋势,叶绿素含量显著降低,且‘巴马火麻’中含量显著高于‘云麻5号’;大麻的伤害程度随p H胁迫的增加而显著增加;由权重分析可知叶绿素含量、SOD活性、地上部含水量可作为大麻苗期耐盐性评价的重要指标.以上结果可为大麻的实际栽培与应用提供理论支持.
The seedlings of hemp were treated with six kinds of mixed salts composed of NaCl, Na2SO4, Na2CO3 and NaHCO3 at various proportions and differing in pH values.The indicators of morphological growth and physiology were determined to study the effects of different mixed salts on hemp seedlings growth. The results showed that plant height, aboveground dry weight and root length were significantly lower than the control group by the increase of pH, however, root shoot ratios and aboveground water content were significantly higher than that of the control group.SOD activity and MDA content sharply increased, soluble sugar content was increased then of PCA (principal component analysis) indicated that chlorophyll content, aboveground water content, and SOD activity could be used as the leading mixed indexes of salts resistance assessment in the seedling stage of hemp. The results for the cultivation of hemp and application provide a theoretical support.