中文摘要：

目的：采用单次长时程应激模型（single prolonged stress paradigm,SPS）作为创伤后应激障碍模型,综合行为学、免疫荧光技术手段观察杏仁核的激活在大鼠PTSD样症状中的作用。方法：在SPS模型中,先后给予大鼠2 h的束缚,20 min的强迫游泳,休息15 min,最后用乙醚麻醉。利用旷场实验、高架十字迷宫检测PTSD的焦虑样行为学改变。利用免疫荧光技术检测杏仁核的FOS蛋白表达变化,并用来表征其脑区激活。结果：同未造模动物相比,造模后第14 d大鼠在旷场中央区和高架十字的开臂停留时间均显著缩短（P〈0.05）,造模后第1 d大鼠和正常对照组相比却没有差异性改变,表明SPS可诱导大鼠产生延迟性的焦虑样行为。FOS蛋白免疫荧光结果显示在杏仁核脑区,同未造模动物相比,造模14 d后FOS蛋白的表达含量增高（P〈0.05）。结论：大鼠PTSD样症状同时伴随有杏仁核的激活,这可能参与了PTSD样行为的发生。

英文摘要：

Objective： The single-prolonged stress（ SPS） as an animal model of post-traumatic stress disorders（ PTSD） was used to explore the role of amygdala activation in the development of PTSD syndrome.Methods： SPS procedure consisted of 3 stages： restraint for 2 h,forced swimming for 20 min,and ether anesthesia. Open field（ OF） and elevated plus maze（ EPM） were used to examine for the anxiety-like behaviors. Immunofluorescence was used to examine the expression of FOS in amygdala.Results： The results of OF test showed that the time spent in central area of OF and open arms /（ open arms ＋ closed arm） index of EPM were significantly reduced in the 14 d SPS group（ both P〈0. 05）,comparing with that in the groups of no SPS and SPS 1d. The results of immunofluorescence analyses showed that FOS expression levels in amygdala in the group of 14 d after SPS groups were significantly increased（ P〈0. 05）,comparing with that of the other two groups.Conclusion： SPS induced PTSD-like behaviors may be associated with the increased activation of amygdala.