中文摘要：

PC12细胞培养过程中添加镉和/或PI3K抑制剂LY294002,免疫印迹法检测Beclin-1复合物和LC3-II的表达变化,研究其在自噬中的作用。结果表明：20μmol·L-1镉处理不同时间后,Beclin-1、class III PI3K和Bcl-2的表达总体呈现先升高后降低的趋势;联合LY294002后显著抑制了Beclin-1,class III PI3K、Bcl-2和LC3-II的表达水平（P〈0.05或P〈0.01）。说明镉暴露PC12细胞后,促使Bcl-2/Beclin-1复合物解离,Beclin-1进一步和class III PI3K结合,参与调节自噬。

英文摘要：

To investigate the effect of Beclin-1compounds in cadmium-induced autophagy,relevant proteins of Beclin-1complex and LC3-II were examined by immunoblot analysis after PC-12 cells were treated with 20μmol·L-1 Cd and/or LY294002,a PI3 Kinhibitor for different times.Results showed that,in cells treated with 20μmol·L-1 Cd for 0-24 h,the protein levels of Beclin-1,class III PI3 Kand Bcl-2were firstly upregulated then gradually decreased.LY294002 significantly reduced the expression of Beclin-1,class III PI3 K,Bcl-2and LC3-II,compared to cadmium alone（P〈 0.05 or P 〈0.01）.This finding suggested that the exposure of Cd facilitated the release of beclin-1from Bcl-2/beclin-1,allowing it to form a complex with class III PI3 Krequired for regulating autophagy.