中文摘要：

鸡屎藤次苷甲酯是中药栀子中含量较高的环稀醚萜类成分之一，目前对其在肝微粒中的代谢研究较少。为更好的阐明鸡屎藤次苷甲酯在不同种属肝微粒体中的代谢差异，本研究应用超高效液相色谱串联高分辨静电场轨道阱质谱技术（UPLC-OribitrapMS／MS），结合鸡屎藤次苷甲酯的电喷雾电离源质谱裂解规律，快速鉴定并比较了鸡屎藤次苷甲酯在大鼠、比格犬、恒河猴和人4种肝微粒体中的代谢产物及主要代谢产物的生成比例差异。通过代谢产物的一级及二级碎片的精确分子量信息，共鉴定出5个代谢产物。结果显示，鸡屎藤次苷甲酯在肝微粒体中的主要代谢途径为水解、氧化和还原反应，且种属间存在一定的差异性，该研究为进一步阐明环稀醚萜类成分的体内代谢途径提供了研究基础。

英文摘要：

In order to clarify the metabolism pathways of scandoside methyl ester, the analysis of metabolites profiling in four kinds of liver microsomes was performed by using an ultra-performance liquid chromatography/ electrospray-tandem mass spectrometry （UPLC-ESI-MS）. The data obtained from the 0 h-incubation and the 2 h-incubation were compared and analyzed. After incubation, 5 metabolites of scandoside methyl ester were found in rat, Beagles, rhesus monkey and human liver microsome. The results showed that scandoside methyl ester＇s major metabolic pathway in the liver microsomes is hydrolysis, oxidation and reduction reactions, and there are certain kinds differences between species. The study provides a research base for further research about iridoid compounds in vivo metabolic pathways.