东篱科研大数据发现系统（DRDS）

位置：成果数据库 > 期刊 > 期刊详情页

Effect of La^3＋ on osteoblastic differentiation of rat bone marrow stromal cells

ISSN号：2096-0328
期刊名称：《康复学报》
时间：0
分类：R336[医药卫生—人体生理学;医药卫生—基础医学]
作者机构：[1]Department of Chemical Biology, Peking University School of Pharmaceutical Sciences, Beijing 100083, China, [2]Department of Chemistry, Huazhong University of Science and Technology, Wuhan 430074, China
相关基金：Acknowledgements This work was supported by the National Natural Science Foundation of China （Grant No. 20031010）.

作者： LIU Hongmei[1,2], ZHANG Tianlan[1], XU Shanjin[1], WANG Kui[1]

关键词：稀土离子, 骨髓基质细胞, 成骨细胞, 大鼠, La^3＋, marrow stromal cells, differentiation.

中文摘要：

在现在的工作，象相关机制一样的主要老鼠骨头髓 stromal 房间(MSC ) 的 osteoblastic 区别上的 La~(3+) 的效果被学习。区别被碱性磷酸(高山) 的察觉监视活动， osteocalcinsecretion，类型Ⅰ骨胶原和 osteocalcin 的 mRNA 层次，和矩阵矿化作用。La~(3+) 禁止的 Theresults 表演 MSC 在的 osteoblastic 区别早并且文化的中世纪由高山活动的减少示威了 osteocalcin 分泌物， osteocalcin 的 mRNA 水平的 anddown 规定。然而，， La~(3+) 不在先进 MSC 影响矩阵矿化作用它 mRNA 类型Ⅰ骨胶原铺平，并且支持的起来调整在在文化的迟了的阶段的 MSC 的高山活动和 osteocalcin 分泌物。另外，西方的污点分析展出那 La~(3+) 导致磷酸化作用和激活激活 ofmitogen 的蛋白质激(MAPK ) 。而且， MAPK 激禁止者 PD98059 完全在文化的中间的阶段在 MSC 的高山活动堵住 La~(3+) 的禁止的效果。这些结果建议 La~(3+) 影响取决于区别舞台的 MSC osteoblastic 区别。La~(3+) 由 MAPK 依赖的机制在早、中间的阶段禁止 MSC 的 osteoblastic 区别，但是不在先进 MSC 影响矩阵矿化作用。

英文摘要：

In the present work, the effect of La^3＋ on osteoblastic differentiation of primary rat bone marrow stromal cells （MSCs） as well as the related mechanisms are studied. Differentiation is monitored by detection of alkaline phosphatase （ALP） activity, osteocalcin secretion, the mRNA levels of Type Ⅰ collagen and osteocalcin, and matrix mineralization. The results show that La^3＋ inhibits osteoblastic differentiation of MSCs in the early and middle stages of culture, as demonstrated by the decrease of ALP activity, osteocalcin secretion, and down-regulation of the mRNA level of osteocalcin. However, La^3＋ does not affect the matrix mineralization in advanced MSCs, because it up-regulates the mRNA levels of Type Ⅰ collagen, and promotes ALP activity and osteocalcin secretion in MSCs in the late stage of culture. In addition, Western blot analysis exhibits that La^3＋ induces the phosphorylation and activation of mitogen-activated protein kinase （MAPK）. Furthermore, MAPK kinase inhibitor PD98059 completely blocks the inhibitory effect of La3＋ on ALP activity of MSCs in the middle stage of culture. These results suggest that La^3＋ affects MSCs osteoblastic differentiation depending on differentiation stages. La3＋ in- hibits osteoblastic differentiation of MSCs in the early and middle stages by a MAPK-dependent mechanism, but does not affect the matrix mineralization in advanced MSCs.

同期刊论文项目