中文摘要：

在视网膜 / 脉络膜的 Retinoic 酸水平在导致的近视模型被改变。All-trans-retinol 脱氢酶(RDH8 ) 是 retinoic 酸新陈代谢的重要的酶。这研究试图与高近视调查 RDH8 基因的协会。三单个核苷酸多型性( SNP )[ RDH851 ( rs2233789 )， RDH8E5a ( rs1644731 )，和 RDH855b ( rs3760753 )]被选择基于从以前的研究的 RDH8 的连接不平衡模式，并且为 160 个汉中国原子家庭与的 genotyped 高度近视(? 10 屈光度或更坏)象在有 166 个高度近视的案例的一个独立的组一样的后代(? 10 屈光度或更坏)并且 211 控制。基于家庭的协会分析用基于家庭的协会测试(FBAT ) 被执行包裹，和遗传型亲戚风险(GRR ) 用 GenAssoc 节目被计算。基于人口的协会分析用 Chi 平方测试被执行。这些 SNP 在与对方一起的连接平衡。SNP RDH851 (rs2233789 ) 和 RDH8E5a (rs1644731 ) 两个都没与高近视显示出协会。SNP RDH855b (rs3760753 ) 与 0.543 的 GRR 表明了重要协会(P=0.0269 )(95% 信心 interval=0.3040.968， P=0.038 ) 。然而，协会在多重比较修正以后变得统计上不足道。Haplotype 分析也没显示出一个重要协会。基于人口的协会分析也没显示出重要协会(P > 0.05 ) 。我们的基于家庭、基于人口的数据两个都建议 RDH8 基因是不大可能的用汉语与高近视被联系。

英文摘要：

Retinoic acid level in the retina/choroid is altered in induced myopia models.All-trans-retinol dehydrogenase（RDH8） is an important enzyme of retinoic acid metabolism.This study aimed to investigate the association of the RDH8 gene with high myopia.Three single nucleotide polymorphisms（SNPs） [RDH851（rs2233789） ,RDH8E5a（rs1644731） ,and RDH855b（rs3760753） ]were selected,based on the linkage disequilibrium pattern of RDH8 from a previous study,and genotyped for 160 Han Chinese nuclear families with highly myopic（-10 diopters or worse） offspring as well as in an independent group with 166 highly myopic cases（-10 diopters or worse） and 211 controls. Family-based association analysis was performed using the family-based association test（FBAT） package,and genotype relative risk（GRR） was calculated using the GenAssoc program.Population-based association analysis was performed using Chi-square test.These SNPs were in linkage equilibrium with each other.SNPs RDH851（rs2233789） and RDH8E5a（rs1644731） both did not show association with high myopia.SNP RDH855b（rs3760753） demonstrated significant association（P=0.0269） with a GRR of 0.543（95%confidence interval=0.304-0.968,P=0.038） .The association became statistically insignificant,however,after multiple comparison correction.Haplotype analysis did not show a significant association either.Population-based association analysis also showed no significant association（P〉0.05） .Our family-and population-based data both suggest that the RDH8 gene is unlikely to be associated with high myopia in Chinese.