从瑞香狼毒根际这一独特的生态环境中采用平板稀释涂布法分离出一株高抑菌活性的真菌,经形态学特征、ITS序列分析及与马尔尼菲青霉菌的比较分析表明,该菌为疣孢青霉菌YL-52(Penicilliumverrucu-losum YL-52),其Genbank注册序列号为EU914140。抑菌活性试验显示,其发酵液粗提物具抑菌广谱性,对细菌和植物病原真菌都有一定的抑制作用,其中对苹果干腐、苹果腐烂、番茄早疫病菌的抑制率达到80%以上。乙酸乙酯相TLC法系统预试结果表明,其主要成分为挥发油、黄酮、萜类、甾体及其苷类、酚性成分、内酯及香豆素和有机酸类。
By streak plate method, a strain of high antifungal active microorganism was isolated from the particular ecological environment-the rhizosphere of Stellera chamaejasme. Morphological character istics, sequences analysis of ITS and comparation analysis with Penicillium marneffei revealed that it belonged to the Penicilliurn verruculosum YL-52,its GenBank accession number is EU914140. Resist ant experiment showed that the extract of fermentative liquid could resist many microorganisms. Its inhibition rates to Fusarium bulbigenum, Valsa mali, Alternario solani reached above 80%. The TLC systemic pretes of ethyl acetate extract showed that the main ingredients are volatile oils, flavonoids, terpins , steroid saponins and its glycosides,phenils,cumarins and lactones, organic acids.