在酸缓冲液,有正电荷的蛋白质能在同步轻散布的大改进(SLS ) 与阴离子表面活化剂和结果反应信号。在这贡献,关联词实验被做把人的血清白蛋白(HSA ) 和免疫球蛋白 G (IgG ) 的相互作用与钠十二烷基 sulfonate (SDS ) 作比较。基于散布信号的极化光的大小,散布极化的一个新方法被组成与分子量差别区分这二个相互作用系统(HSA 66 kDa;IgG 150kDa ) 。结果与数据(DLS ) 由动态轻散布测量了一致技术。
In acid buffer solution, proteins with positive charge can react with anion surfactsnt and result in a great enhancement of synchronous light scattering (SLS) signals. In this contribution, the correlative experiment was made to compare the interaction of human serum albumin (HSA) and immunoglobuIin G (IgG) with sodium dodecyl sulfonate (SDS). Based on the measurements of the polarization light scattering signals, a new method of scattering polarization was constituted to distinguish these two interaction systems with molecular weight difference (HSA 66 kDa; IgG 150 kDa). The results were consistent with the data measured by dynamic light scattering (DLS) technique.