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牛支原体临床分离株牛体毒力和免疫原性比较

ISSN号：1007-5038
期刊名称：《动物医学进展》
时间：0
分类：S852.62[农业科学—基础兽医学;农业科学—兽医学;农业科学—畜牧兽医]
作者机构：[1]华中农业大学农业微生物国家重点实验室,湖北武汉430070, [2]华中农业大学动物医学院,湖北武汉430070, [3]华中农业大学动物科技学院,湖北武汉430070
相关基金：国家公益性行业（农业）科研专项（201003060）; 宁夏科学技术厅重大科技专项（宁夏肉牛呼吸疾病综合征及皮肤病防控技术研究与示范）; 现代农业（肉牛）产业技术体系专项资金（CARS-38）

作者：张瑞[1,2], 崔朋[1,2], 巴晓亮[1,2], 汪漫[1,2], 韩潇潇[1,2], 祁晶晶[1,2], 彭清洁[2], 陈曦[1,2], 胡长敏[1,2], 陈颖钰[3], 郭爱珍[1,2]

关键词：牛支原体, 牛, 人工感染, 毒力, 免疫, Mycoplasma bovis, cattle, artificial challenge, virulence, immunization

中文摘要：

牛支原体肺炎是我国肉牛的新现传染病,目前尚无特异性预防措施。本研究比较3株牛支原体临床分离株和1株ATCC参考株PG45的牛体毒力和免疫原性,筛选免疫原性良好的强毒株,为牛支原体灭活疫苗研究提供侯选菌株。6月龄左右本地健康黄牛经气管注射相同剂量的牛支原体,连续观察22d后处死,记录攻毒前后试验牛的临床症状,血液白细胞变化,体重变化,特异性细胞免疫和体液免疫反应等;剖检观察病理变化和组织病理变化,并用免疫组化方法检测组织中抗原量。4株牛支原体菌株中,临床分离株XY毒力最强,导致牛体重减轻,临床症状明显,大体和组织病理变化严重,同时能诱导较好的免疫反应。然而,各株牛支原体抗原在感染牛肺和淋巴结的分布没有显著差异。通过牛体试验,筛选出1株牛支原体灭活疫苗研究侯选菌株,同时为牛支原体毒力研究和疫苗效力评价提供了牛体感染模型。

英文摘要：

Mycoplasma bovis pneumonia is an emerging infectious disease of beef cattle.Up to now,there is no specific control tool to prevent this disease.By comparing the virulence and immunogenicity of three clinically isolated M.bovis strains and reference strain PG45 in cattle,this study was aimed to screen the most promising candidate strain for development of an inactivated vaccine against M.bovis.The 15 6-month old calves were injected intratraceally with equal doses of M.bovis strains for 3 continuous days and observed for 22 days.The samples（nasal swabs and blood） were collected at a regular interval.The parameters recorded and tested included clinical signs,rectal temperature,daily weight gain,blood cell counts,serum IgA and IgG titers,levels of serum IFN-β and TNF-α.The gross and histopathological lesions were scored and antigen distribution in lung and lymph node tissues was detected with immunohistochemistry.Among the four M.bovis strains,the XY strain has the strongest virulence shown by loss of weight,stunted growth,serious gross and histopathological lesions to greatest extent and induction of the highest immune response.However,the antigen distribution among the strains did not produce any difference.The present study not only obtained a candidate strain of M.bovis for development of inactivated vaccine,but also provided a good infection model for M.bovis virulence research and vaccine efficacy evaluation.

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