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广东省桔小实蝇种群感染Wolbachia的分子检测
  • 分类:Q966[生物学—昆虫学]
  • 作者机构:[1]仲恺农业工程学院外来有害生物预警与控制研究所,广州510225, [2]仲恺农业工程学院生命科学学院,广州510225, [3]广东出入境检验检疫局检验检疫技术中心,广州510623
  • 相关基金:国家自然科学基金项目(30671378); 广东省自然科学基金(07301292)
中文摘要:

为探究不同桔小实蝇Bactrocera dorsalis(Hendel)地理种群的遗传变异、入侵来源和扩散情况,利用13对引物对中国南方10省区、泰国、夏威夷、菲律宾和老挝的30个桔小实蝇种群共180个个体的遗传多样性水平进行了研究。Popgene32和NTSYS-pc2.10e软件分析结果表明:30个不同桔小实蝇种群的遗传相似度在0.3599~0.9153范围内。种群的Nei氏基因多样性指数平均为0.6464±0.1026,Shannon信息指数I平均为1.2845±0.2632,提示桔小实蝇种群具有较丰富的遗传多样性。UPGMA聚类分析显示,福建地区和海南地区分别独立一支,广东地区和台湾地区种群聚成一支,而广西、泰国、湖南、云南、老挝、四川、重庆和贵州地区聚为一大支系。据此提出泰国种群和老挝种群是最早入侵我国的种群,云南地区是最早的入侵地,广西地区可能为又一较早入侵地。

英文摘要:

To explore the genetic variation,the source of invasion and the dispersal of different geographical populations of Bactrocera dorsalis(Hendel),the genetic diversity and relationships of 30 B.dorsalis populations consisting of 180 individuals collected from 10 provinces of South China,Thailand,Hawaii,Philippines,and Laos were analyzed with 13 microsatellite loci.The analysis results with the softwares of Popgene32 and NTSYS-pc2.10e showed that the Nei's genetic identity of 30 B.dorsalis populations was between 0.3599 and 0.9153.A relatively high level of genetic diversity was revealed:H=0.6464±0.1026,I=1.2845±0.2632.UPGMA cluster analysis indicated that the populations from Fujian and Hainan gathered separately into two different clades,the populations from Guangdong and Taiwan gathered into one clade,while the populations from Guangxi,Thailand,Hunan,Yunnan,Laos,Sichuan,Chongqing and Guizhou formed the other one.So it is inferred that the populations from Thailand and Laos were the earliest ones to invade China,Yunnan was the region to be invaded earliest,and Guangxi was probably also among the regions to be invaded early by this pest.

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