中文摘要：

以黄河流域棉花品种鲁棉6号为试验材料，以易再生的棉花品系YZ1为对照，通过不同质量浓度的激素组合成功诱导鲁棉6号产生体细胞胚并发育成苗。结果表明：所用的12种激素组合均能有效诱导出愈伤组织，其中以1.0 mg/L IBA或0.1 mg/L 2,4-D和0.2 mg/L KT组合的诱导效果最佳；愈伤组织继代时培养基中生长素质量浓度减半，KNO3质量浓度加倍；挑选黄绿色或灰绿色、质地疏松的颗粒状愈伤继代于0.01 mg/L 2,4-D和0.1 mg/L KT 或0.1 mg/L IBA且KNO3用量加倍的培养基中，可在1~2个月内产生胚性愈伤组织，胚性愈伤组织再转到分化培养基上可产生体细胞胚；体细胞胚在1/2 MS培养基中进一步发育成再生小苗。利用该组织培养再生系统，鲁棉6号和YZ1都能在6~8个月内成功获得再生植株。组织学观察表明，IBA处理下鲁棉6号胚性愈伤组织细胞主要来源于棉花下胚轴初生形成层的细胞。

英文摘要：

An efficient somatic embryogenesis system was developed to regenerate plantlets from hypocotyls of upland cotton cultivars Lumian 6 （Gossypium hirsutum L. ）. The cultivar Lumian 6 was cultivated in North China,the same cotton planting region as YZl,an easy-going genotype somatic em- bryogenesis. Calli were produced on the medium with twelve different hormone regimes, the hormone re- gimes 1.0 mg/L IBA only or 0. 1 mg/L 2,4-D together with O. 2 mg/L KT were the best for inducing calli from Lumian 6. After about 4 weeks, the peripheral cultures were separated from explants and transferred to fresh MS medium with half auxin and doubled KNOa. After two to three subcultures （30 days intervals）, friable, yellow-green or grey-green embryogenic cultures and somatic embryos was effec- tively observed in ECM media with low concentration of auxin （0. 01 mg/L 2,4-D and 0. 1 mg/L KT or 0.1 mg/L IBA only）. After being transferred to the media for differentiation and embryo-germination, somatic embryos were produced and further developed. The two varieties can regenerate plantlets in 6-8 months using the protocols mentioned above. Histological observation demonstrated that the majority of embryogenic calli originated in a familiar way in the two genotypes and proved that the cells from prima- ry cambium cells were the original cells for forming cotton embryogenic calli.