中文摘要：

中国短吻鳄，短吻鳄 sinensis，是极其危及的种。为危及的种的基因资源的一个保存工程首先包含器官，纸巾，配偶子， genomic DNA 图书馆和细胞线的保藏。现在的学习是第一建立， cryopreserve 房间从中国短吻鳄肝，心和肌肉纸巾排队。学习表明在在在肝(1112 d ) 之中的主要文化的房间迁居时间有大差异，心(1314 d ) 和肌肉(1718 d ) 组织片。差别及时在主要房间文化建议为爬行动物造内脏导出房间线是相对容易的。生物分析证明为融化的房间的人口双倍工资是约 36 h。因为 2n=32 是 88.6%93.4% ， Karyotyping 表明显示出染色体的中国短吻鳄房间的频率数。这里建立的中国短吻鳄房间线为研究提供一个重要资源并且是可能的为这稀罕、极其危及的种的保护有用。而且，这些方法的建立可以为为另外的爬行动物种类在细胞的水平保存基因资源供应技术、理论的支持。

英文摘要：

The Chinese alligator, Alligator sinensis, is a critically endangered species. A conservation project of gene resources for an en- dangered species first involves the preservation of organs, tissues, gametes, genomic DNA libraries and cell lines. The present study is the first to establish and cryopreserve cell lines of liver, heart and muscle tissues from the Chinese alligator. The study revealed that there was a large discrepancy in cell migration time in primary cultures among liver （11-12 d）, heart （13-14 d） and muscle （17-18 d） tissue pieces. The differences in time in primary cell culture suggested that it was relatively easy to build visceral-derived cell lines for reptiles. Biological analysis showed that the population doubling time for thawed cells was approximately 36 b. Karyotyping revealed that the frequency of Chinese alligator cells showing chromosome number as 2n=32 was 88.6%-93.4%. Chinese alligator cell lines established here provide a vital resource for research and are likely to be useful for protection of this rare and critically endangered species. Furthermore, the establishment of these methods may supply technical and theoretical support for preserving genetic resources at the cellular level for other reptile species.