中文摘要：

基于非手性离子液体对手性配体交换的促进作用，建立了-种测定去氧肾上腺素手性异构体的毛细管电泳分离分析方法。在系统优化了电泳条件后，采用20kV的分离电压、25℃的毛细管柱温、254nm的检测波长以及5s的压力（3447Pa）进样时间，在添加4．0mmol／LCu（Ⅱ）、8．0mmol／LL-脯氨酸（L—Pro）和15mmol／L氯化-1-丁基-3-甲基咪唑（[BMIM]C1）的20mmol／L Tris—H3PO4缓冲溶液（pH5．4）中，R-去氧肾上腺素和g去氧肾上腺素的分离度为1．42，峰面积与质量浓度分别在12．5～150．0mg／L和15．0～150．0mg／L范围内有线性关系。将该方法用于加标血液和尿液样品中R和S型去氧肾上腺素的测定，尿液中的加标回收率为93．7％～108．2％，相对标准偏差在3．18％（n=3）以内；血液中的加标回收率为91．4％-113．1％，相对标准偏差在4．82％（n=3）以内。

英文摘要：

A method for the separation and determination of optical isomers of phenylephrine was developed based on the promoting effect of non-chiral ionic liquid on chiral ligand- exchange capillary electrophoresis after the electrophoretic parameters were optimized system- atically. R-phenylephrine and S-phenylephrine can be separated and determined effectively in 20 mmol/L Tris-H3PO4 buffer solution （pH 5.4） composed of 4.0 mmol/L Cu（ II ）, 8.0 mmol/L L-proline （L-Pro） and 15 mmol/L !-butyl-3-methylimidazolium chloride （ [ BMIM 1 C1） with the applied voltage of 20 kV, capillary temperature of 25 0（;, detection wavelength of 254 nm, and injection of 5 s at 3 447 Pa. The resolution of R- and S-phenylephrines was 1.42. The linear ran- ges for the determination of R-phenylephrine and S-phenylephrine were 12.5- 150 mg/L and 15.0- 150 mg/L, respectively. The method has been satisfactorily used for the determination of R-phenylephrine and S-phenylephrine in the spiked blood and urine samples. The spiked recov- eries in the urine sample were in the range of 93.7% - 108.2% with the RSDs lower than 3. 18% （ n = 3 ）, and the spiked recoveries in the blood sample were in the range of 9 1. 4% and [ 13. 1% with the RSDs lower than 4.82% （ n = 3）.